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MABS2045-100UG Anti-HPa2 Antibody, clone DHIC2-2B4

Detects Alpha cells of human pancreatic islets using this mouse monoclonal Anti-HPa2, clone DHIC2-2B4, Cat. No. MABS2045, tested for use in Immunohistochemistry (Paraffin) and Flow Cytometry.

Anti-HPa2 Antibody, clone DHIC2-2B4 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling UniProt Number: NA
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MABS2045-100UG
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIH(P), FCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS2045-100UG
      DescriptionAnti-HPa2 Antibody, clone DHIC2-2B4
      Alternate Names
      • Human pancreas alpha cells
      Background InformationPancreas performs both the endocrine and the exocrine function. The bulk of pancreas is composed of exocrine cells that produce digestion enzymes which are released into ducts to be emptied into the duodenum. The endocrine pancreas is composed of islets of Langerhans that produce hormones. At least five distinct endocrine cell types have been identified in the endocrine pancreas, each producing a different hormone. Beta cells produce insulin, which is considered as an anabolic hormone and regulates blood glucose levels and is also involved in fat and protein synthesis. Alpha cells produce glucagon that acts when blood glucose levels are low. Ductal cells of pancreas form the epithelial lining of the branched tubes that deliver digestive enzymes into the duodenum. They also secrete bicarbonate to neutralize acidic contents coming in from stomach. Duct cells may contribute up to 10% of all pancreatic cells. Acinar cells function as a unit of the exocrine pancreas and they synthesize, store, and secrete digestive enzymes (lipase, alpha amylase, trypsin, and chymotrypsin), which under normal physiological conditions are activated only after reaching the duodenum. Premature activation of these enzymes within pancreatic acinar cells can lead to acute pancreatitis. Clone DHIC2-2B4 can be used to detect alpha cells in human pancreatic islets. (Ref.: Dorrell, C., et al. (2008). Stem Cell Research (2008). 1(3); 183-194; Grapin-Botton, A (2005). Int. J. Biochem. Cell Biol. 37(3); 504-510; Leung, PS (2006). Int. J. Biochem. Cell Biol. 38(7); 1024-1030).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationDetects Alpha cells of human pancreatic islets using this mouse monoclonal Anti-HPa2, clone DHIC2-2B4, Cat. No. MABS2045, tested for use in Immunohistochemistry (Paraffin) and Flow Cytometry.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Flow Cytometry
      Application NotesImmunohistochemistry Analysis: A representative lot detected HPa2 in Immunohistochemistry applications (Dorrell, C., et. al. (2008). Stem Cell Res. 1(3):183-94).

      Flow Cytometry Analysis: A representative lot detected HPa2 in Flow Cytometry applications (Dorrell, C., et. al. (2008). Stem Cell Res. 1(3):183-94; Bramswig, N.C., et. al. (2013). J Clin Invest. 123(3):1275-84; Dorrell, C., et. al. (2016). Nat Commun. 7:11756).
      Biological Information
      ImmunogenTrypsin-dissociated human pancreatic islet cells containing low levels of exocrine and ductal cells.
      CloneDHIC2-2B4
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone DHIC2-2B4 specifically detects alpha cells in human pancreatic tissue sections.
      IsotypeIgG1λ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Purification MethodProtein G purified
      UniProt Number
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in alpha cells of human pancreatic tissue sections.

      Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected HPa2 in alpha cells of human pancreatic tissue sections.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS2045-100UG 04054839529658