Millipore Sigma Vibrant Logo

ABF202 Anti-IRF7 Antibody

This Anti-IRF7 Antibody is validated for use in Western Blotting and Immunohistochemistry and Immunofluorescence for the detection of IRF7.

Anti-IRF7 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Immunological Signaling Gene Symbol: IRF7 UniProt Number: Q92985
View Products on Sigmaaldrich.com
ABF202
100 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
—
Request Pricing
—
Limited Availability Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Overview

      Replacement Information

      Special Offers

      100% Performance Guaranteed

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, IHC, IFRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABF202
      DescriptionAnti-IRF7 Antibody
      Alternate Names
      • Interferon regulatory factor 7
      • IRF7
      Background InformationInterferon regulatory factor 7 (IRF7) is a member of the IRF family and has 1 IRF tryptophan pentad repeat DNA-binding domain. IRF7 is key transcriptional regulator of type I interferon (IFN)-dependent immune responses and regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. IRF7 plays an important role in the innate immune response against DNA and RNA viruses and has been shown to activate both the IFN-beta (IFNB) and the IFN-alpha (IFNA) genes and mediate their induction via both the virus-activated, MyD88-independent pathway and the TLR-activated, MyD88-dependent pathway. IRF7 exists in an inactive form in the cytoplasm of uninfected cells and following viral infection it becomes phosphorylated by IKBKE and TBK1 kinases. This leads to its dimerization and nuclear localization where along with other coactivators it can activate transcription of the type I IFN and ISG genes. Studies show that IRF7 induces PSMB9/LMP2 expression, either directly or through induction of IRF1 and may play a role in regulating adaptive immune responses. Other evidence shows that IRF7 binds to the Q promoter (Qp) of EBV nuclear antigen 1 a (EBNA1) and may play a role in the regulation of EBV latency. Additionally, IRF7 is implied to activate distinct gene expression programs in macrophages and regulate the anti-tumor properties of primary macrophages. IRF7 is expressed predominantly in spleen, thymus and peripheral blood leukocytes.
      References
      Product Information
      FormatAffinity Purified
      Control
      • HEK293 whole cell lysate
      PresentationPurified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-IRF7 Antibody is validated for use in Western Blotting and Immunohistochemistry and Immunofluorescence for the detection of IRF7.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Immunofluorescence
      Application NotesImmunohistochemistry Analysis: 5 µg/mL from a representative lot detected IRF7 in mouse spleen tissue.

      Immunofluorescence Analysis: 20 µg/mL from a representative lot detected IRF7 in mouse spleen cells.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the C-terminus of human IRF7.
      EpitopeC-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes the C-terminus of IRF7.
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Gene Symbol
      • IRF7
      Purification MethodAntigen Affinity Purified
      UniProt Number
      Molecular Weight~51 kDa observed. Uncharacterized bands may appear in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293 whole cell lysate.

      Western Blotting Analysis: 2 µg/mL of this antibody detected IRF7 in HEK293 whole cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABF202 04053252923425