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AB9754-I-25UG Anti-Kisspeptin-10

Anti-Kisspeptin-10, Cat. No. AB9754-I, is a rabbit polyclonal antibody that detects Metastasis-suppressor KiSS-1 and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

AB9754-I-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberAB9754-I-25UG
      DescriptionAnti-Kisspeptin-10
      Alternate Names
      • Kisspeptin-10
      • Metastasis-suppressor KISS-1
      • Kisspeptin-1
      • KISS1
      Background InformationMetastasis-suppressor KISS-1 (UniProt: Q15726; also known as Kisspeptin-1) is encoded by the KISS1 (also known as PP5098) gene (Gene ID: 3814) in human. KISS1 is highly expressed in placenta and some expression is also observed in testis, pancreas, liver, small intestine. Low expression levels are also observed in the brain. Its expression is shown to increase in both early placentas and molar pregnancies and are reduced in choriocarcinoma cells. Its expression is reported to be higher levels in first trimester trophoblasts than at term of gestation. KISS-1 is a secreted metastasis suppressor that demonstrates inhibition of metastases formation in several types of cancers. It is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off to generate the mature form that can also be cleaved into four chains known as Metastin (aa 68-121), Kisspeptin-14 (aa 108-121), Kisspeptin-13 (aa 109-12), and Kisspeptin-10 (112-121). Metastin acts as an endogenous ligand of GPR54, a GPCR. Activation of this receptor inhibits cell proliferation and cell migration that contributes to its tumor suppressor role. Kisspeptin-10 is reported to be a paracrine/endocrine regulator in fine-tuning trophoblast invasion generated by the trophoblast itself and increases intracellular Ca2+ levels in isolated first trimester trophoblasts. KISS1 expression is reported to be significantly reduced in human pancreatic cancer tissue and this reduced expression is associated with advanced disease. KISS1 also diminishes MMP9 expression by effecting reduced NF-kB binding to the promoter. (Ref.: McNally, LR., et al. (2010). Clin. Exp. Metastasis. 27(8); 591-600). D3
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide
      Quality LevelMQ200
      Applications
      ApplicationAnti-Kisspeptin-10, Cat. No. AB9754-I, is a rabbit polyclonal antibody that detects Metastasis-suppressor KiSS-1 and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Kisspeptin-10 in human placenta tissue sections.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 10 amino acids from the C-terminal region (Kisspeptin-10).
      EpitopeC-terminal half
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Kisspeptin. It targets an epitope within Kisspeptin-10 at the C-terminal region.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Monkey based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • KISS1
      • PP5098
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~16 kDa observed; 14.71 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HL-60 cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected Kisspeptin-10 in HL-60 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AB9754-I-25UG 04065265085953