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MABF2072-25UG Anti-MAdCAM-1 Antibody, clone MECA-367

Anti-MAdCAM-1, clone MECA-367, Cat. No. MABF2072, is a rat monoclonal antibody that detects murine Mucosal addressin cell adhesion molecule 1 (MAdCAM-1) and has been tested for use in Immunohistochemistry (Paraffin), Inhibition/Function assays, Neutralizing, and Western Blotting.

Anti-MAdCAM-1 Antibody, clone MECA-367 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: Madcam1 UniProt Number: Q61826
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MABF2072-25UG
25 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MIH(P), INHIB, NEUT, WBRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABF2072-25UG
      DescriptionAnti-MAdCAM-1 Antibody, clone MECA-367
      Alternate Names
      • Mucosal addressin cell adhesion molecule 1
      • mMAdCAM-1
      Background InformationMucosal addressin cell adhesion molecule 1 (UniProt: Q61826; also known as MAdCAM-1, mMAdCAM-1) is encoded by the Madcam1 gene (Gene ID: 17123) in murine species. MAdCAM-1 is a cell adhesion leukocyte receptor that helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It is highly expressed on high endothelial venules (HEV) of organized intestinal lymphoid tissues and mesenteric lymph nodes, and in the lamina propria of the intestine. Its expression is also observed in brain endothelioma cells and mucosal tissues during chronic inflammation. Aberrant expression of MAdCAM-1 has also been reported in the inflamed pancreas. MAdCAM-1 can bind both the integrin alpha-4/beta-7 and L-selectin and is involved in regulating both the passage and retention of leukocytes. It is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off in mature form. MAdCAM-1 has an extracellular domain (aa 22-109), a transmembrane domain (aa 365-385), and a cytoplasmic domain (aa 386-405). It also contains three Ig-like domains (aa 22-109; 110-227; 258-357) and a Mucin-like region (aa 221-257). Two isoforms of MAdCAM-1 have been described that are produced by alternative splicing. Both isoforms can adhere to integrin alpha-4/beta-7. Isoform 2 that lacks the mucin-like domain is believed to be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding. Interaction between integrin alpha 4 and MAdCAM-1 is considered to be important for the homing of diabetogenic T cells to the pancreas. (Ref.: Savinov, AY., et al. (2003). J. Exp. Med. 197(5); 643-656; Rivera-Nieves, J., et al. (2005). J. Immunol. 174(4); 2343-2352).
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2a in PBS without azide.
      Applications
      ApplicationAnti-MAdCAM-1, clone MECA-367, Cat. No. MABF2072, is a rat monoclonal antibody that detects murine Mucosal addressin cell adhesion molecule 1 (MAdCAM-1) and has been tested for use in Immunohistochemistry (Paraffin), Inhibition/Function assays, Neutralizing, and Western Blotting.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Inhibits Activity/Function
      • Neutralizing
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected MAdCAM-1 in Western Blotting applications (Streeter, P.R., et. al. (1988). Nature. 331(6151):41-6).

      Immunohistochemistry (Paraffin) Analysis: A representative lot detected MAdCAM-1 in Immunohistochemistry applications (Streeter, P.R., et. al. (1988). Nature. 331(6151):41-6).

      Immunohistochemistry Analysis: A representative lot detected MAdCAM-1 in Immunohistochemistry applications (Streeter, P.R., et. al. (1988). Nature. 331(6151):41-6; Rantakari, P., et. al. (2016). Nature. 538(7625):392-396).

      Neutralizing Analysis: A representative lot neutralizes the action on MAdCAM-1 and reduces chronic ileitis (Rivera-Nieves, J., et. al. (2005). J Immunol. 174(4):2343-52).


      Inhibits Activity/Function: A representative lot almost completely inhibited lymphocyte migration to Peyer s patches and also blocked the adhesion of the mucosal high endothelial venules (HEV) binding to T cell lymphoma TK1. (Streeter, P.R., et. al. (1988). Nature. 331(6151):41-6; Briskin, M.J., et. al. (1993). Nature. 363(6428):461-4).
      Biological Information
      ImmunogenEndothelial cells isolated from BALB/c mouse mesenteric and peripheral lymph nodes.
      CloneMECA-367
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityClone MECA-367 detects Mucosal addressin cell adhesion molecule 1 (MAdCAM-1) in murine cells.
      IsotypeIgG2aκ
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Madcam1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight43.65 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in mouse colon tissue sections.

      Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected MAdCAM-1 in mouse colon tissue sections.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF2072-25UG 04054839516696