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MABS1202 Anti-MKK7/SKK4 Antibody, clone 3F6.5

This Anti-MKK7 Antibody, clone 3F6.5 is validated for use in Western Blotting, Immunocytochemistry for the detection of SKK4.

Anti-MKK7/SKK4 Antibody, clone 3F6.5 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: MAP Kinases Gene Symbol: MAP2K7, JNKK2, MEK7, MKK7, PRKMK7, SKK4 UniProt Number: O14733
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MABS1202
100 μg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1202
      DescriptionAnti-MKK7/SKK4 Antibody, clone 3F6.5
      Alternate Names
      • Dual specificity mitogen-activated protein kinase kinase 7
      • c-Jun N-terminal kinase kinase 2
      • MAP kinase kinase 7
      • MAPK/ERK kinase 7
      • MAPKK 7
      • MEK 7
      • JNK-activating kinase 2
      • JNK kinase 2
      • JNKK 2
      • SAPK kinase 4
      • SAPKK-4
      • SAPKK4
      • Stress-activated protein kinase kinase 4
      Background InformationDual specificity mitogen-activated protein kinase kinase 7 (EC 2.7.12.2; UniProt O14733; also known as c-Jun N-terminal kinase kinase 2, MAP kinase kinase 7, MAPK/ERK kinase 7, MAPKK 7, MEK 7, JNK-activating kinase 2, JNK kinase 2, JNKK 2, SAPK kinase 4, SAPKK-4, SAPKK4, Stress-activated protein kinase kinase 4) is encoded by the MAP2K7 (also known as JNKK2, MEK7, MKK7, PRKMK7, SKK4) gene (Gene ID 5609) in human. MKK7 belongs to the family of mitogen-activated protein kinase kinases (MAPKKs) involved in mediating cellular signal transduction in response to proinflammatory cytokines stimulation and environmental stresses. MKK7 and the related MKK4 function as crucial upstream transducers in the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway by phosphorylating different JNK isoforms. MKK7 is activated by upstream MKKKs, whereby the MKKKs interact with MKK7 through its C-terminal DVD domain (a.a. 377-400) and activate MKK7 by phosphorylating serine and threonine residues at a S-K-A-K-T motif located in the MKK7 kinase domain (a.a. 120-380). Upon activation, MKK4 and MKK7 in turn phosphorylate specific tyrosine and threonine residues of the conserved T-P-Y motif in the activation loop of substrate JNK proteins. The N-terminal D domain (a.a. 37-57), consisting of a cluster of positively charged amino acids followed by alternating hydrophobic amino acids, is required for the recruitment of MAPKK substrates, such as JNK. Alternative splicing results in four isoforms. Isoform B (beta), gamma, and isoform 4 differ from the canonical isoform A (alpha) by a 43-a.a. expansion at a.a. 111, a 16-a.a. expansion at a.a. 42, and a 7-a.a. expansion at a.a. 312, respectively, of the MKK7alpha sequence.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-MKK7 Antibody, clone 3F6.5 is validated for use in Western Blotting, Immunocytochemistry for the detection of SKK4.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesWestern Blotting Analysis: 1 µg/mL from a representative lot detected MKK7/SKK4 in 10 µg of NIH/3T3 cell lysate.
      Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected MKK7/SKK4 in HeLa, HepG2, and NIH/3T3 cells.
      Biological Information
      ImmunogenGST-tagged recombinant human MKK7/SKK4 internal fragment.
      EpitopeInternal (N-terminal half).
      Clone3F6.5
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 3F6.5 was raised against recombinant N-terminal fragment derived from human MKK7/SKK4 spliced isoform 1 (isoform A or alpha) and isoform 4. The corresponding region in mouse isoform 2 & 8 (isoform alpha & beta 2) differ from the human sequence by only one amino acid (human A69 vs. mouse T69) Cross-reactivity toward other human and murine spliced isoforms is highly expected, but has not been confirmed.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MAP2K7
      • JNKK2
      • MEK7
      • MKK7
      • PRKMK7
      • SKK4
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~50 kDa observed. 47.35/51.75/48.94/48.05 kDa (human isoform 1/2/3/4, Met1 removed), 59.18/47.43/42.59/39.25/44.08/49.02/52.37/50.78 kDa (mouse isoform 1/2/3/4/5/6/7/8, Met1 removed) calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected MKK7/SKK4 in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS1202 04055977353594

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies