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MABC1608-25UG Anti-Mucin-16 (CA-125) Antibody, clone 5E11

Detect Mucin-16 using this mouse monoclonal Anti-Mucin-16 (CA-125), clone 5E11 Antibody, Cat. No. MABC1608-25UG, validated for use in Immunohistochemistry (Paraffin) and Western Blotting, Proximity Ligation Assay.

Anti-Mucin-16 (CA-125) Antibody, clone 5E11 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: MUC16, CA125 UniProt Number: Q8WXI7
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MABC1608-25UG
25 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIH(P), WB, Proximity Ligation AssayMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC1608-25UG
      DescriptionAnti-Mucin-16 (CA-125) Antibody, clone 5E11
      Alternate Names
      • MUC-16
      • Ovarian cancer-related tumor marker CA125
      • CA-125
      • Ovarian carcinoma antigen CA125
      Background InformationMucin-16 (UniProt: Q8WXI7; also known as MUC-16, Ovarian cancer-related tumor marker CA125, CA-125, Ovarian carcinoma antigen CA125) is encoded by the MUC16 (also known as CA125) gene (Gene ID: 94025) in human. Mucin-16 is a heavily O-glycosylated protein that can be liberated into the extracellular space following the phosphorylation of the intracellular C-terminus, which induces proteolytic cleavage and liberation of extracellular domain. I provides protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Mucin-16 is composed of three domains: a Serine, Threonine-rich N-terminal domain, a repeated domain containing between 12 and 60 partially conserved tandem repeats of 156 amino acids and a C-terminal transmembrane domain with a short cytoplasmic tail. Its expression is up-regulated in ovarian cancer cells and may serve as a marker for ovarian cancer. Mucin-16 is shown to binds to mesothelin (MSLN) and this binding mediates heterotypic cell adhesion, which may contribute to the metastasis of ovarian cancer to the peritoneum by initiating cell attachment to the mesothelial epithelium.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Mucin-16 using this mouse monoclonal Anti-Mucin-16 (CA-125), clone 5E11 Antibody, Cat. No. MABC1608-25UG, validated for use in Immunohistochemistry (Paraffin) and Western Blotting, Proximity Ligation Assay.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      • Proximity Ligation Assay
      Application NotesImmunohistochemistry Analysis: A representative lot detected Mucin-16 in ovarian serous cystadenocarcinoma tissue (C ourtesy of Dr. Leonor David, M.D., Ph.D., IPATIMUP and Medical Faculty of the University of Porto, Portugal).
      Proximity Ligation Assay Analysis: A representative lot detected Mucin-16 in Proximity Ligation Assay applications (Ricardo, S., et. al. (2016). Virchows Arch. 468(6):715-22; Ricardo, S., et. al. (2015). Mol Oncol. 9(2):503-12).
      Immunohistochemistry Analysis: A representative lot detected Mucin-16 in Immunohistochemistry applications (Marcos-Silva, L., et. al. (2015). Glycobiology. 25(11):1172-82; Ricardo, S., et. al. (2015). Mol Oncol. 9(2):503-12).
      Immunohistochemistry Analysis: A representative lot detected Mucin-16 in ovarian serous cystadenocarcinoma tissue (C ourtesy of Dr. Leonor David, M.D., Ph.D., IPATIMUP and Medical Faculty of the University of Porto, Portugal).
      Western Blotting Analysis: A representative lot detected Mucin-16 in Western Blotting applications (Marcos-Silva, L., et. al. (2015). Glycobiology. 25(11):1172-82).
      Biological Information
      ImmunogenHis-tagged recombinant fragment corresponding to 264 amino acids from the C-terminal half of human Mucin-16.
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 5E11 detects Mucin-16 in human ovarian cancer tissue. It targets an epitope within 264 amino acids from the C-terminal half.
      IsotypeIgG1
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • MUC16
      • CA125
      Purification MethodProtein G purified
      UniProt Number
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in human ovarian cancer and human uterine tissues.

      Immunohistochemistry (Paraffin) Analysis: A 1:50-250 dilution of this antibody detected Mucin-16 in human ovarian cancer and human uterine tissues.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABC1608-25UG 04054839437885