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MABT893 Anti-Platelet Antibody, clone PAC-1

Anti-Platelet, clone PAC-1, Cat. No. MABT893 is a mouse monoclonal antibody that detects Integrin alpha-IIb and has been tested for use in Dot Blot, ELISA, Flow Cytometry, Radioimmunoassay, and Western Blotting.

Anti-Platelet Antibody, clone PAC-1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Gene Symbol: ITGA2B, GP2B, ITGAB UniProt Number: P08514
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MABT893
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HDB, ELISA, FC, RIA, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT893
      DescriptionAnti-Platelet Antibody, clone PAC-1
      Alternate Names
      • Integrin alpha-IIb
      • GPalpha IIb
      • GPIIb
      • Platelet membrane glycoprotein IIb
      • CD41)
      Background InformationIntegrin alpha-IIb (UniProt: P08514; also known as GPalpha IIb, GPIIb, Platelet membrane glycoprotein IIb, CD41) is encoded by the ITGA2B (also known as GP2B, ITGAB) gene (Gene ID: 3674) in human. GPIIb is a single-pass type I membrane protein of the integrin alpha chain family. It serves as a receptor of fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. . Following activation it brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation, which physically plugs ruptured endothelial cell surface. GPIIb is synthesized with a signal peptide of 31 amino acids that is cleaved to produce the mature form that has an extracellular domain (aa 32-993), a helical domain (aa 994-1019), and a cytoplasmic tail (aa 1020-1039). Three isoforms of GPII b are reported that are generated by alternative splicing. Isoform 1 and 2 have been identified in platelets and megakaryocytes, but not in reticulocytes or in Jurkat and U-937 white blood cell line. Isoform 3 is expressed by leukemia, prostate adenocarcinoma and melanoma cells but not by platelets or normal prostate or breast epithelial cells. Clone PAC- 1 is a pentameric IgM that is reported to bind to the activated platelets, but not to unstimulated platelets.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgM in PBS with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Platelet, clone PAC-1, Cat. No. MABT893 is a mouse monoclonal antibody that detects Integrin alpha-IIb and has been tested for use in Dot Blot, ELISA, Flow Cytometry, Radioimmunoassay, and Western Blotting.
      Key Applications
      • Dot Blot
      • ELISA
      • Flow Cytometry
      • Radioimmunoassay
      • Western Blotting
      Application NotesDot Blot Analysis: A representative lot detected Platelet in Dot Blot applications (Shattil, S.J., et. al. (1985). J Biol Chem. 260(20):11107-14).

      ELISA Analysis: A representative lot detected Platelet in ELISA applications (Shattil, S.J., et. al. (1985). J Biol Chem. 260(20):11107-14).

      Radioimmunoassay Analysis: A representative lot detected Platelet in Radioimmunoassy applications (Shattil, S.J., et. al. (1985). J Biol Chem. 260(20):11107-14; Shattil, S.J., et. al. (1987). Blood. 70(1):307-15).

      Western Blotting Analysis: A representative lot detected Platelet in Western Blotting applications (Shattil, S.J., et. al. (1985). J Biol Chem. 260(20):11107-14).

      Flow Cytometry Analysis: A representative lot detected Platelet in Flow Cytometry applications (Caron, A., et. al. (2002). J Cardiovasc Pharmacol. 40(2):296-306; Taub, R., et. al. (1989). J Biol Chem. 264(1):259-65; Shattil, S.J., et. al. (1987). Blood. 70(1):307-15).

      Flow Cytometry Analysis: A 1:200 dilution from a representative lot detected Platelet in freshly isolated human platelets activated with convulxin (Courtesy of Shuchi Gupta, PhD, University of Pennsylvania, USA).
      Biological Information
      ImmunogenPlatelets obtained from the blood of a patient with Glanzmann's thrombasthenia.
      ClonePAC-1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone PAC-1 is a pentameric IgM that binds to the activated platelets, but not to unstimulated platelets.
      IsotypeIgMκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ITGA2B
      • GP2B
      • ITGAB
      UniProt Number
      Molecular Weight113.38 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceIsotype testing: Identity Confirmation by Isotyping Test.

      Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgM.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABT893 04054839354663