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AB12226 Anti-RALY Antibody

Use Anti-RALY Antibody (rabbit polyclonal antibody) validated in WB, IP to detect RALY also known as hnRNP core protein C-like 2, RNA-binding protein (autoantigenic hnRNP-associated with lethal yellow).

Anti-RALY Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: RNA Metabolism & Binding Proteins Gene Symbol: P542, HNRPCL2, RALY UniProt Number: Q9UKM9
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AB12226
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, IPRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB12226
      DescriptionAnti-RALY Antibody
      Alternate Names
      • RNA binding protein, autoantigenic (hnRNP-associated with lethal yellow homolog (mouse))
      • RNA-binding protein (autoantigenic)
      • hnRNP associated with lethal yellow protein homolog
      • hnRNP core protein C-like 2
      • RNA-binding protein (autoantigenic, hnRNP-associated with lethal yellow)
      • Heterogeneous nuclear ribonucleoprotein C-like 2
      • RNA binding protein (autoantigenic, hnRNP-associated with lethal yellow)
      • Autoantigen p542
      Background InformationRNA-binding protein RALY is a member of the RRM HNRPC family and contains a characteristic RRM (RNA recognition motif) domain. It is thought to participate in pre-mRNA splicing and may be a heterogenous nuclear ribonucleoprotein. Phosphorylation of RALY has been observed following damage to DNA. Expression has been observed in brain, heart, kidney, liver, lung, pancreas, and skeletal muscle.
      References
      Product Information
      FormatAffinity Purified
      Control
      • HepG2 cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationUse Anti-RALY Antibody (rabbit polyclonal antibody) validated in WB, IP to detect RALY also known as hnRNP core protein C-like 2, RNA-binding protein (autoantigenic hnRNP-associated with lethal yellow).
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesImmunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated RALY from HepG2 cell lysate.
      Biological Information
      ImmunogenHistidine-tagged recombinant protein corresponding to human RALY.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryIn infectious mononucleosis, anti-EBNA-1 antibodies are produced which cross-react with multiple normal human proteins. The cross-reactivity is due to anti-gly/ala antibodies that cross-react with host proteins containing configurations like those in the EBNA-1 repeat. One such antigen is RALY which is a member of the heterogeneous nuclear ribonucleoprotein gene family. [provided by RefSeq].
      Gene Symbol
      • P542
      • HNRPCL2
      • RALY
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Probable-RNA binding protein. Could be a heterogeneous nuclear ribonucleoprotein (hnRNP). May be involved in pre-mRNA splicing (By similarity).

      SUBUNIT STRUCTURE: Identified in the spliceosome C complex, at least composed of AQR, ASCC3L1, C19orf29, CDC40, CDC5L, CRNKL1, DDX23, DDX41, DDX48, DDX5, DGCR14, DHX35, DHX38, DHX8, EFTUD2, FRG1, GPATC1, HNRPA1, HNRPA2B1, HNRPA3, HNRPC, HNRPF, HNRPH1, HNRPK, HNRPM, HNRPR, HNRPU, KIAA1160, KIAA1604, LSM2, LSM3, MAGOH, MORG1, PABPC1, PLRG1, PNN, PPIE, PPIL1, PPIL3, PPWD1, PRPF19, PRPF4B, PRPF6, PRPF8, RALY, RBM22, RBM8A, RBMX, SART1, SF3A1, SF3A2, SF3A3, SF3B1, SF3B2, SF3B3, SFRS1, SKIV2L2, SNRPA1, SNRPB, SNRPB2, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, SNW1, SRRM1, SRRM2, SYF2, SYNCRIP, TFIP11, THOC4, U2AF1, WDR57, XAB2 and ZCCHC8.

      SUBCELLULAR LOCATION: Nucleus Probable.

      TISSUE SPECIFICTY: Expressed in heart, brain, lung, liver, skeletal muscle, kidney and pancreas. Weakly expressed in placenta.

      PTM: Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.6 Ref.9 Ref.10 Ref.11 Ref.12 Ref.13 Ref.14 Ref.15 Ref.16 Ref.17 Ref.19 Ref.20

      MISCELLANEOUS: Autoantigen found in infectious mononucleosis caused by Epstein-Barr virus. An epitope recognized by B-cells, which cross-react with the BKRF1 protein (EBNA-1 nuclear protein) of Epstein-Barr virus has been identified.

      SEQUENCE SIMILARITIES: Belongs to the RRM HNRPC family. RALY subfamily.

      Contains 1 RRM (RNA recognition motif) domain.
      Molecular Weight~ 38 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HepG2 cell lysate.

      Western Blot Analysis: 1:10,000 dilution of this antibody detected RALY on 10 µg of HepG2 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AB12226 04053252415920