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MABF3085-100UG Anti-SARS-CoV-2 Spike RBD Antibody, clone 1A4-3F8

Anti-SARS-CoV-2 Spike RBD, clone 1A4-3F8, Cat. No. MABF3085, is a mouse monoclonal antibody that detects the receptor binding domain of SARS-CoV-2 spike protein and is tested for use in Immunofluorescence, Immunoprecipitation, and Western Blotting.

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MABF3085-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF3085-100UG
      DescriptionAnti-SARS-CoV-2 Spike RBD Antibody, clone 1A4-3F8
      Alternate Names
      • Spike glycoprotein
      • S glycoprotein
      • E2
      • Peplomer protein
      Background InformationSpike glycoprotein (UniProt: P0DTC2; also known as S glycoprotein, E2, Peplomer protein) is encoded by the S (also known as 2) gene (Gene ID: 43740568) in Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The SARS-CoV-2 is a positive-strand RNA virus that causes severe respiratory syndrome in human. The mature SARS-CoV-2 contains 4 structural proteins: Envelope (E), Membrane (M), Nucleocapsid (N), and the Spike protein (S). E and M proteins help in viral assembly and N protein is needed for RNA synthesis. The S protein is a single-pass type I, homotrimeric, membrane glycoprotein that is responsible for virus binding and entry into host cell. It is synthesized with a signal peptide (aa 1-12), which is subsequently cleaved off to generate the mature protein that contains an extracellular domain (aa 13-1213), a transmembrane domain (aa 1214-1234), and a cytoplasmic domain 1235-1273). The S protein is further processed into S1 (aa 13-685) and S2 (aa 686-1273) subunits by host cell furin. The presence of a furin polybasic cleavage site in S protein from SARS-CoV-2 sets it apart from S protein in SARS-CoV that possesses a monobasic S1/S2 cleavage site. The S1 subunit has the receptor binding domain (RBD; aa 319-541) that mediates entry of SARS-CoV-2 into sensitive cells through the peptidase domain of host Angiotensin-converting enzyme 2 (ACE2) with high affinity (KD = 15 nM). The S2 domain (aa 686-1213), which is reported to be well conserved, is responsible for membrane fusion.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-SARS-CoV-2 Spike RBD, clone 1A4-3F8, Cat. No. MABF3085, is a mouse monoclonal antibody that detects the receptor binding domain of SARS-CoV-2 spike protein and is tested for use in Immunofluorescence, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunofluorescence
      Application NotesTested Applications

      Immunoprecipitation Analysis: 2 µg from a representative lot immunoprecipitated SARS-CoV-2 spike glycoprotein in HEK293T S-P2A-GFP (Data courtesy of Dr. Stefan Schüchner, Dr. Egon Ogris, Max Perutz Labs, Medical University of Vienna, Austria).

      Western Blotting Analysis: 0.1 µg from a representative lot detected SARS-CoV-2 spike glycoprotein in lysates from HEK293T cells expressing full-length spike protein of SARS-CoV-2 (Data courtesy of Dr. Stefan Schüchner, Dr. Egon Ogris, Max Perutz Labs, Medical University of Vienna, Austria).

      Immunofluorescence Analysis: A representative lot detected SARS-CoV-2 spike glycoprotein in HEK293T cells expressing full-length spike protein of SARS-CoV-2 (Data courtesy of Dr. Stefan Schüchner, Dr. Egon Ogris, Max Perutz Labs, Medical University of Vienna, Austria).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenHis-tagged recombinant fragment from the C-terminal region of the receptor binding domain (RBD) of SARS-CoV-2 Spike protein.
      EpitopeC-terminal
      Clone1A4-3F8
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 1A4-3F8 is a mouse monoclonal antibody that specifically detects receptor binding domain (RBD) of SARS-CoV-2 Spike protein. It targets an epitope within the C-terminal region of RBD.
      IsotypeIgG1κ
      Species Reactivity
      • Virus
      Species Reactivity NoteSARS-CoV-2
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • S
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~180 kDa observed; 141.18 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in lysate from HEK293T cells expressing full-length SARS-CoV-2 spike protein.

      Western Blotting Analysis (WB): A 1:250 dilution of this antibody detected SARS-CoV-2 spike glycoprotein in lysate from HEK293T cells expressing full-length SARS-CoV-2 spike protein.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF3085-100UG 04065268529027