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MABN2433-25UG Anti-TMEM16B Antibody, clone 13B8

Anti-TMEM16B, clone 13B8, Cat. No. MABN2433, is a highly specific mouse monoclonal antibody that targets Transmembrane protein 16B (Anoctamin-2) and has been tested in Immunofluorescence and Western Blotting.

Research Category: Neuroscience Gene Symbol: Ano2, Tmem16b UniProt Number: Q8CFW1
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MABN2433-25UG
25 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MIF, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN2433-25UG
      DescriptionAnti-TMEM16B Antibody, clone 13B8
      Alternate Names
      • Anoctamin-2
      • Transmembrane protein 16B
      Background InformationAnoctamin-2 (UniProt: Q8CFW1; also known as Transmembrane protein 16B, TMEM16B) is encoded by the Ano2 (also known as Tmem16b) gene (Gene ID: 243634) in murine species. TMEM16B is a multi-pass membrane protein that serves as a calcium-activated chloride channel (CaCC) that plays a role in olfactory signal transduction and also mediates light perception amplification in retina. Its channel activity is shown to be repressed by chloride channel inhibitors, such as niflumic acid and flufenaic acid. TMEM16 B is expressed in retina, especially in the photoreceptor synaptic terminals, and in olfactory epithelium, particularly in sensory neurons (OSNs) and cilia. It is also shown to be expressed in retinal pigment epithelium (RPE), olfactory bulb, and cortex. It is detected in the mantle layer of the neural tube and in the dorsal root ganglia at E14.5. TMEM16B is also involved in a calcium-dependent mode of ionic plasticity that reduces the efficacy of GABAergic synapses. It is reported to attenuate GABAergic transmission by increasing the postsynaptic chloride concentration, hence reducing the driving force for chloride influx.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-TMEM16B, clone 13B8, Cat. No. MABN2433, is a highly specific mouse monoclonal antibody that targets Transmembrane protein 16B (Anoctamin-2) and has been tested in Immunofluorescence and Western Blotting.
      Key Applications
      • Immunofluorescence
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected TMEM16B in mouse retina tissue (Courtesy of Dr. Robert Molday, Ph.D., University of British Columbia, Canada).

      Immunofluorescence Analysis: A representative lot detected TMEM16B in mouse retina tissue (Courtesy of Dr. Robert Molday, Ph.D., University of British Columbia, Canada).
      Biological Information
      ImmunogenGST-tagged recombinant fragment corresponding to the first 74 amino acids of murine Transmembrane protein 16B.
      Clone13B8
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 13B8 detects Transmembrane protein 16B (TMEM16B) in murine neural tissue.
      IsotypeIgG1κ
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • Ano2
      • Tmem16b
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight130 kDa observed; 114.13 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in mouse retinal tissue.
      Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected TMEM16B in mouse retinal tissue.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN2433-25UG 04054839522260

      Documentation