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AB3831-I Anti-phospho Cofilin-1 Antibody (Ser3)

This rabbit polyclonal Anti-phospho Cofilin-1 (Ser3), Cat. No. AB3831-I is validated for use in Peptide Inhibition Assay and Western Blotting for the detection of Cofilin-1 Ser3 phosphorylation.

Anti-phospho Cofilin-1 Antibody (Ser3) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Gene Symbol: CFL, CFL1 UniProt Number: P23528
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AB3831-I
100 µL  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, PIARbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB3831-I
      DescriptionAnti-phospho Cofilin-1 Antibody (Ser3)
      Alternate Names
      • Cofilin-1, Ser3-phosphorylated
      • 18 kDa phosphoprotein, Ser3-phosphorylated
      • Cofilin, non-muscle isoform, Ser3-phosphorylated
      Background InformationCofilin-1 (UniProt P23528; also known as 18 kDa phosphoprotein, Cofilin non-muscle isoform, p18) is encoded by the CFL1 (also known as CFL) gene (Gene ID 1072) in human. Cofilin-1 (non-muscle cofilin), cofilin-2 (muscle cofilin), and actin-depolymerizing factor (ADF) play an essential role in regulating actin filament dynamics and reorganization by stimulating the severance and depolymerization of actin filaments. The three cofilin family members share 70% to 81% sequence identity, exhibit similar biological functions, and display distinct but overlapping expression patterns. Cofilins and ADF bind actin monomers and filaments (G-actin and F-actin) and increase the number of actin monomers and filament fragments, facilitating filament turnover and treadmilling at key locations in migrating cells. Cofilins and ADF are inactivated by phosphorylation at Ser3 by LIM-kinases (LIMKs) and testicular protein kinases (TESKs) and are reactivated by dephosphorylation by the slingshot (SSH) family of protein phosphatases and chronophin. Cfl1-knockout results in mouse embryonic lethality at E11.5 12.5 due to aberrant neural tube closure and defective neural crest cell migration, while Adf-deficient mice are viable with normal brain appearance, but suffer from corneal defects that cause blindness in adult mice.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis rabbit polyclonal Anti-phospho Cofilin-1 (Ser3), Cat. No. AB3831-I is validated for use in Peptide Inhibition Assay and Western Blotting for the detection of Cofilin-1 Ser3 phosphorylation.
      Key Applications
      • Western Blotting
      • Peptide Inhibition Assay
      Application NotesPeptide Inhibition Assay: Target band detection in 10 µg of HeLa cell lysate was prevented by preblocking a representative lot with the immunogen phosphopeptide, but not the corresponding non-phosphopeptide.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the N-termnal sequence of human cofilin-1 with phosphorylated Ser3.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityTarget band detection by this polyclonal antibody was prevented by preblocking with the immunogen phosphopeptide, but not the corresponding non-phosphopeptide.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Bovine, Mouse, Porcine, Rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CFL
      • CFL1
      Modifications
      • Phosphorylation
      Purification MethodAffinity purified.
      UniProt Number
      Molecular Weight~19 kDa observed. 18.39/18.37/184.3/18.39/18.40 kDa (bovine/human/mouse/porcine/rat) calculated (Met1 removed). Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: A 1:200 dilution of this antibody detected Ser3-phosphorylatd cofilin-1 in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AB3831-I 04054839086182