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MABE1821-25UG Anti-phospho-HDAC1 (Ser406) Antibody, clone BT-15

Anti-phospho-HDAC1 (Ser406), clone BT-15 Antibody, Cat. No. MABE1821, is a highly specific mouse monoclonal antibody that targets HDAC1 phosphorylated on serine 406 and has been tested foer use in Immunocytochemistry, Immunoprecipitation. and Western Blotting.

MABE1821-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABE1821-25UG
      DescriptionAnti-phospho-HDAC1 (Ser406) Antibody, clone BT-15
      Alternate Names
      • Histone deacetylase 1
      • HD1
      Background InformationHistone deacetylase 1 (UniProt: Q13547; also known as EC:3.5.1.98, HD1, HDAC1) is encoded by the HDAC1 (also known as RPD3L1) gene (Gene ID: 3065) in human. HDAC1 is a ubiquitous enzyme that is expressed at higher levels in heart, pancreas, and testis and lower levels in kidney and the brain. It is a part of the core histone deacetylase complex that is composed of HDAC1, HDAC2, RBBP4 and RBBP7. HDAC1 is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) and this deacetylation gives a tag for epigenetic repression. It plays an important role in transcriptional regulation, cell cycle progression, and developmental events. HDAC1 is also a component of the BRG1-RB1-HDAC1 complex that negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREB binding protein is recruited, which facilitates transcriptional activation. Phosphorylation of HDAC1 is shown to stabilize its interactions with binding partners in multiprotein complexes. Phosphorylation on serine 421 and serine 423 are constitutively phosphorylated by casein kinase II to regulate its activity. Clone BT-15 specifically detects HDAC1 phosphorylated on serine 406 in the prophase state, which is critical for cells to prepare for mitosis and marks the onset of events for cell division. (Ref.: Segre, CV., et al. (2016). MAbs. 8(1):37-42).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-phospho-HDAC1 (Ser406), clone BT-15 Antibody, Cat. No. MABE1821, is a highly specific mouse monoclonal antibody that targets HDAC1 phosphorylated on serine 406 and has been tested foer use in Immunocytochemistry, Immunoprecipitation. and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation Analysis: A representative lot immunoprecipitated phospho-HDAC1 (Ser406) in Immunoprecipitation applications (Segre, C.V., et. al. (2016). MAbs. 8(1):37-42).

      Immunocytochemistry Analysis: A representative lot detected phospho-HDAC1 (Ser406) in Immunocytochemistry applications (Segre, C.V., et. al. (2016). MAbs. 8(1):37-42).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 19 amino acids surrounding phosphoserine 406 from the C-terminal region of human Histone deacetylase 1.
      CloneBT-15
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone BT-15 is a mouse monoclonal antibody that specifically detects Histone deacetylase phosphorylated on serine 406 in mitotic synchronized cells. It targets an epitope within 19 amino acids from the C-terminal region.
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Bovine, Monkey, Rat, Rabbit based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HDAC1
      • RPD3L1
      Modifications
      • Phosphorylation
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight60 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in lysate from HeLa cells pretreated with anti-mitotic agent (Nocodazole).

      Western Blotting Analysis: 2 µg/mL of this antibody detected phospho-HDAC1 (Ser406) in lysates from HeLa cells pretreated with Nocodazole (330 nM for 16 h).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE1821-25UG 04054839010378

      Documentation

      Anti-phospho-HDAC1 (Ser406) Antibody, clone BT-15 MSDS

      Title

      Safety Data Sheet (SDS) 

      Anti-phospho-HDAC1 (Ser406) Antibody, clone BT-15 Certificates of Analysis

      TitleLot Number
      Anti-phospho-HDAC1 (Ser406), clone BT-15 - 3253065 3253065

      References

      Reference overviewPub Med ID
      A monoclonal antibody specific for prophase phosphorylation of histone deacetylase 1: a readout for early mitotic cells.
      Segré, CV; Senese, S; Loponte, S; Santaguida, S; Soffientini, P; Grigorean, G; Cinquanta, M; Ossolengo, G; Seiser, C; Chiocca, S
      MAbs  9  37-43  2017

      Show Abstract       		26467746 26467746