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16-193 Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301, biotin conjugate

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICC, WBMBiotinMonoclonal Antibody
      Catalogue Number16-193
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301, biotin conjugate
      Alternate Names
      • H2AXS139P
      • Histone H2A.X (phospho S139)
      • H2A histone family, member X
      • H2AX histone
      Background InformationHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.
      Product Information
      • Staurosporine-treated Jurkat cell lysates.
      PresentationPurified biotin conjugated mouse monoclonal IgG1 in buffer containing 70% storage buffer (0.02 M Phosphate buffer, pH7.6, 0.25 M NaCl, 0.1% sodium azide) and 30% glycerol
      Quality LevelMQ100
      ApplicationAnti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301, biotin conjugate is a Mouse Monoclonal Antibody for detection of phospho-Histone H2A.X (Ser139) also known as H2AXS139P, H2AX histone & has been validated in ICC & WB.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide (CKATQA[pS]QEY) corresponding to amino acids 134-142 of human histone H2A.X. The immunizing sequence has 8 identical amino acids in yeast and mouse.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityRecognizes Histone H2A.X phosphorylated at Ser139, Mr 15kDa.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. A broad species cross-reactivity is expected based on conservation of sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.
      Gene Symbol
      • H2AFX
      • H2AX
      • H2a/x
      • H2A/X
      • H2A.X
      • Phosphorylation
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P16104 # Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C- terminal phosphorylation.
      SIZE: 143 amino acids; 15145 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Interacts with numerous proteins required for DNA damage signaling and repair when phosphorylated on Ser-140. These include MDC1, TP53BP1, BRCA1 and the MRN complex, composed of MRE11A, RAD50, and NBN. Interaction with the MRN complex is mediated at least in part by NBN. Also interacts with DHX9/NDHII when phosphorylated on Ser-140.
      DEVELOPMENTAL STAGE: Synthesized in G1 as well as in S-phase.
      DOMAIN: SwissProt: P16104 The [ST]-Q motif constitutes a recognition sequence for kinases from the PI3/PI4-kinase family.
      PTM: Phosphorylated on Ser-140 (to form gamma-H2AFX) in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks, and may also occur during meiotic recombination events and immunoglobulin class switching in lymphocytes. Phosphorylation can extend up to several thousand nucleosomes from the actual site of the DSB and may mark the surrounding chromatin for recruitment of proteins required for DNA damage signaling and repair. Widespread phosphorylation may also serve to amplify the damage signal or aid repair of persistent lesions. Phosphorylation of Ser-140 in response to ionizing radiation is mediated by both ATM and PRKDC while defects in DNA replication induce Ser-140 phosphorylation subsequent to activation of ATR and PRKDC. Dephosphorylation of Ser-140 by PP2A is required for DNA DSB repair. In meiosis, Ser-140 phosphorylation may occur at synaptonemal complexes during leptotene as an ATM-dependent response to the formation of programmed DSBs by SPO11. Ser-140 phosphorylation may subsequently occurs at unsynapsed regions of both autosomes and the XY bivalent during zygotene, downstream of ATR and BRCA1 activation. Ser-140 phosphorylation may also be required for transcriptional repression of unsynapsed chromatin and meiotic sex chromosome inactivation (MSCI), whereby the X and Y chromosomes condense in pachytene to form the heterochromatic XY-body. During immunoglobulin class switch recombination in lymphocytes, Ser-140 phosphorylation may occur at sites of DNA-recombination subsequent to activation of the activation-induced cytidine deaminase AICDA. & Monoubiquitination of Lys-120 by RING1 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression (By similarity).
      SIMILARITY: Belongs to the histone H2A family.
      Molecular Weight15 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated in acid extracted histone from Jurkat cell lysates treated with 0.5mM staurosporine (19-123).

      Western Blot Analysis:
      0.5-1 μg/mL of this lot detected phosphorylated histone H2A.X (Ser139) in acid extracted histone from Jurkat cell lysates treated with 0.5μM staurosporine (Catalog # 19-123).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Global Trade Item Number
      Catalogue Number GTIN
      16-193 04053252284601


      Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301, biotin conjugate MSDS


      Safety Data Sheet (SDS) 

      Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301, biotin conjugate Certificates of Analysis

      TitleLot Number
      Anti-phospho-Histone H2A.X (S139), clone JBW301, biotin conjugate (mouse monoclonal IgG1) - DAM1460180 DAM1460180
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301, biotin conjugate 3027131
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301, biotin conjugate - 2147312 2147312
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301, biotin conjugate - 2342868 2342868
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301, biotin conjugate - 2388944 2388944
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301, biotin conjugate - 2028742 2028742
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301, biotin conjugate - 22238 22238
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301, biotin conjugate - 2287717 2287717
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301, biotin conjugate - 3224640 3224640
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301, biotin conjugate - 32503 32503


      Reference overviewApplicationPub Med ID
      Down-regulation of HtrA1 activates the epithelial-mesenchymal transition and ATM DNA damage response pathways.
      Wang, N; Eckert, KA; Zomorrodi, AR; Xin, P; Pan, W; Shearer, DA; Weisz, J; Maranus, CD; Clawson, GA
      PloS one  7  e39446  2012

      Show Abstract
      22761798 22761798
      Quantitative model of cell cycle arrest and cellular senescence in primary human fibroblasts.
      Schäuble, S; Klement, K; Marthandan, S; Münch, S; Heiland, I; Schuster, S; Hemmerich, P; Diekmann, S
      PloS one  7  e42150  2012

      Show Abstract
      22879912 22879912
      γ-H2AX detection in peripheral blood lymphocytes, splenocytes, bone marrow, xenografts, and skin.
      Christophe E Redon,Asako J Nakamura,Olivier Sordet,Jennifer S Dickey,Ksenia Gouliaeva,Brian Tabb,Scott Lawrence,Robert J Kinders,William M Bonner,Olga A Sedelnikova
      Methods in molecular biology (Clifton, N.J.)  682  2011

      Show Abstract
      21057933 21057933
      RSPO1/β-catenin signaling pathway regulates oogonia differentiation and entry into meiosis in the mouse fetal ovary.
      Chassot, AA; Gregoire, EP; Lavery, R; Taketo, MM; de Rooij, DG; Adams, IR; Chaboissier, MC
      PloS one  6  e25641  2011

      Show Abstract
      21991325 21991325
      Characterization of oxidative guanine damage and repair in mammalian telomeres.
      Wang, Z; Rhee, DB; Lu, J; Bohr, CT; Zhou, F; Vallabhaneni, H; de Souza-Pinto, NC; Liu, Y
      PLoS genetics  6  e1000951  2010

      Show Abstract
      Immunofluorescence20485567 20485567
      Phosphorylation of H2AX at short telomeres in T cells and fibroblasts.
      Hao, LY; Strong, MA; Greider, CW
      The Journal of biological chemistry  279  45148-54  2004

      Show Abstract
      Immunocytochemistry15322096 15322096
      Characterisation of cytotoxicity and DNA damage induced by the topoisomerase II-directed bisdioxopiperazine anti-cancer agent ICRF-187 (dexrazoxane) in yeast and mammalian cells.
      Jensen, LH; Dejligbjerg, M; Hansen, LT; Grauslund, M; Jensen, PB; Sehested, M
      BMC pharmacology  4  31  2004

      Show Abstract
      Western Blotting15575955 15575955

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      Life Science Research > Antibodies and Assays > Primary Antibodies