69042 Sigma-AldrichB834(DE3)pLysS Competent Cells - Novagen
B834 host strains are methionine auxotrophs that allows high specific activity labeling of target proteins with 35S-methionine. T7 lysozyme expression suppresses basal T7 expression.
More>> B834 host strains are methionine auxotrophs that allows high specific activity labeling of target proteins with 35S-methionine. T7 lysozyme expression suppresses basal T7 expression. Less<<B834(DE3)pLysS Competent Cells - Novagen MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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Overview
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| Description | |
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| Overview | B834 is the parental strain for BL21 (1). These protease-deficient hosts are methionine auxotrophs and allow high specific-activity labeling of target proteins with 35S-methionine and selenomethionine for crystallography (2). DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG. pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability. This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com. |
| Genotype: F- ompT hsdSB(rB- mB-) gal dcm met (DE3) pLysS (CamR)
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form. |
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| Catalogue Number | 69042 |
| Brand Family | Novagen® |
| Features and benefits | • Allows for ³⁵S-methionine or selenomethionine labeling for crystallography studies • Deficient in Ion and ompT proteases • Guaranteed transformation efficiencies of >2x10⁶ cfu/µg • Provided in a 0.2 ml volume; each vial can be used for 10 x 20 µL transformations • Prepared using an optimized chemical method |
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| References | 1. Wood, W. B. (1966) J. Mol. Biol. 16, 118–133. 2. Leahy, D. J., Hendrickson, W. A., Aukhil, I., and Erickson, H. P. (1992) Science 258, 987–991. |
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| Components | |
| Guaranteed efficiency | Guaranteed efficiency: > 2 x 10⁶ cfu/μg |
| Quality Level | MQ100 |
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| Ship Code | Dry Ice Only |
| Toxicity | Multiple Toxicity Values, refer to MSDS |
| Storage | ≤ -70°C |
| Avoid freeze/thaw | Avoid freeze/thaw |
| Do not freeze | Ok to freeze |
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| Specifications |
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| Global Trade Item Number | |
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| Catalogue Number | GTIN |
| 69042 | 0 |
Documentation
B834(DE3)pLysS Competent Cells - Novagen MSDS
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B834(DE3)pLysS Competent Cells - Novagen Certificates of Analysis
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| 69042 |
References
| Reference overview |
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| 1. Wood, W. B. (1966) J. Mol. Biol. 16, 118–133. 2. Leahy, D. J., Hendrickson, W. A., Aukhil, I., and Erickson, H. P. (1992) Science 258, 987–991. |
Citations
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User Protocols
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| TB009 Competent Cells |
| TB053 Academic and Non-profit Laboratory Assurance Letter |