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219469 Cell Sheet Migration Inhibitor, Locostatin

The Cell Sheet Migration Inhibitor, Locostatin, also referenced under CAS 133812-16-5, controls the biological activity of (RKIP)/Raf1 kinase. This small molecule/inhibitor is primarily used for Cell Structure applications.

Cell Sheet Migration Inhibitor, Locostatin MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: UIC-1005, (4S)-3-[(E)-But-2-enoyl]-4-benzyl-2-oxazolidinone, Locostatin

Primary Target: (RKIP)/Raf1 kinase
219469
  
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      Overview

      Replacement Information

      Key Spec Table

      CAS #Empirical Formula
      133812-16-5C₁₄H₁₅NO₃
      Description
      Overview

      This product has been discontinued.





      A cell-permeable N-(crotonyl)oxazolidinone compound that acts as a potent inhibitor of Raf kinase inhibitor protein (RKIP)/Raf1 kinase interaction. Shown to block eukaryotic cell migration (IC50 = 14 µM for inhibition of wound closure in MDCK cell monolayers) and decrease formation of lamellipodial protrusions at the wound margin. Also inhibits early development in frog embryos and tissue dynamics in embryonic explants. Displays no antibacterial activity. May serve as a useful tool for studying RKIP-mediated signaling pathways. Although locostatin is an irreversible inhibitor of RKIP, its cellular effects are generally reversible due to new biosynthesis.
      Catalogue Number219469
      Brand Family Calbiochem®
      SynonymsUIC-1005, (4S)-3-[(E)-But-2-enoyl]-4-benzyl-2-oxazolidinone, Locostatin
      References
      ReferencesBement, W.M., et al. 2005. Chem. Biol. 12, 953.
      Zhu, S., et al. 2005. Chem. Biol. 12, 981.
      Mc Henry, K.T., et al. 2002. Chembiochem 3, 1105.
      Product Information
      CAS number133812-16-5
      ATP CompetitiveN
      DeclarationSold under license of U.S. Patent 7,390,826.
      FormWhite solid
      Hill FormulaC₁₄H₁₅NO₃
      Chemical formulaC₁₄H₁₅NO₃
      ReversibleN
      Structure formula ImageStructure formula Image
      Applications
      Biological Information
      Primary Target(RKIP)/Raf1 kinase
      Primary Target IC<sub>50</sub>14 µM for inhibition of wound closure in MDCK cell monolayers
      Purity≥98% by HPLC
      Physicochemical Information
      Cell permeableY
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Carcinogenic / Teratogenic
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      219469 0

      Documentation

      Cell Sheet Migration Inhibitor, Locostatin MSDS

      Title

      Safety Data Sheet (SDS) 

      Cell Sheet Migration Inhibitor, Locostatin Certificates of Analysis

      TitleLot Number
      219469

      References

      Reference overview
      Bement, W.M., et al. 2005. Chem. Biol. 12, 953.
      Zhu, S., et al. 2005. Chem. Biol. 12, 981.
      Mc Henry, K.T., et al. 2002. Chembiochem 3, 1105.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision10-May-2010 RFH
      SynonymsUIC-1005, (4S)-3-[(E)-But-2-enoyl]-4-benzyl-2-oxazolidinone, Locostatin
      DescriptionA cell-permeable and potent inhibitor of Raf kinase inhibitor protein (RKIP)/Raf1 kinase interaction. Shown to block eukaryotic cell migration (IC50 = 14 µM for inhibition of wound closure in MDCK cell monolayers) and decrease formation of lamellipodial protrusions at the wound margin. Also inhibits early development in frog embryos and tissue dynamics in embryonic explants. May serve as a useful tool for studying RKIP-mediated signaling pathways. Although locostatin is an irreversible inhibitor of RKIP, its cellular effects are generally reversible due to new biosynthesis.
      FormWhite solid
      Intert gas (Yes/No) Packaged under inert gas
      CAS number133812-16-5
      Chemical formulaC₁₄H₁₅NO₃
      Structure formulaStructure formula
      Purity≥98% by HPLC
      SolubilityDMSO (5 mg/ml) or Methanol (50 mg/ml)
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Toxicity Carcinogenic / Teratogenic
      ReferencesBement, W.M., et al. 2005. Chem. Biol. 12, 953.
      Zhu, S., et al. 2005. Chem. Biol. 12, 981.
      Mc Henry, K.T., et al. 2002. Chembiochem 3, 1105.