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SCR103 Sigma-AldrichCollagenase type I

The collagenase type I (from Clostridium histolyticum) is a crude collagenase preparation that can be used for the isolation of primary cells or for tissue dissociation by enzymatic means.

Collagenase type I MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
Technical Information

Key Applications: Stem Cell Culture

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SCR103

250 mg 1 vial containing 250mg of lyophilized powder

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Description
Catalogue Number	SCR103
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Collagenase type I
Overview	Collagenase type I (from Clostridium histolyticum) is a crude collagenase preparation that can be used for the isolation of primary cells or for tissue dissociation by enzymatic means. The preparation also may contain caseinase, clostripain, and tryptic activities. This preparation can be used to dissociate cells in culture, and has been successfully used to passage human embryonic stem cells cultured with Millipore’s HEScGROTM Animal Component-Free Medium.
Materials Required but Not Delivered	• Ultrapure water (e.g., MilliQ) • 0.22 μM sterile filtration device (e.g. Millipore’s Stericup-GP, Catalog # SCGPU01RE)

References

Product Information
HS Code	3507 90 19
Formulation
Quality Level	MQ100

Applications
Application	The collagenase type I (from Clostridium histolyticum) is a crude collagenase preparation that can be used for the isolation of primary cells or for tissue dissociation by enzymatic means.
Key Applications	Stem Cell Culture

Biological Information
Media Form	Liquid
Stem Cell Type	Human Embryonic Stem Cells Induced Pluripotent Stem Cells

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Each lot of collagenase type I is assayed via a modification of Mandl (1964), with the extent of collagen breakdown determined using the colorimetric ninhydrin method of Moore and Stein (1948). Each lot is then tested for successful passage of human embryonic stem cells cultured in Millipore's HEScGRO Medium for hES cells. Cells are characterized for correct marker expression and morphology after five passages.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Each lot of collagenase type I is assayed via a modification of Mandl (1964), with the extent of collagen breakdown determined using the colorimetric ninhydrin method of Moore and Stein (1948). Each lot is then tested for successful passage of human embryonic stem cells cultured in Millipore's HEScGRO Medium for hES cells. Cells are characterized for correct marker expression and morphology after five passages.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Collagenase type I should be stored in its powdered form at 2-8oC until ready to use. Solutions of collagenase type I can be aliquotted and stored at -20oC, but should not be repeatedly thawed and re-frozen.

Packaging Information
Material Size	250 mg
Material Package	1 vial containing 250mg of lyophilized powder

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
SCR103	04053252397868

Documentation

Collagenase type I MSDS

Title
Safety Data Sheet (SDS)

Collagenase type I Certificates of Analysis

Title	Lot Number
Collagenase type I	3079679
Collagenase type I	2978576
Collagenase type I	3022083
Collagenase type I	3034146
Collagenase type I	3049470
Collagenase type I	2909011
Collagenase type I	3003794
Collagenase type I	3099012
Collagenase type I	3130613
Collagenase type I -

Technical Info

Title
Collagenase Type I for Enzymatic Passaging of Human Embryonic Stem Cells in HEScGRO Medium

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