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341668 Fibronectin, Rat Plasma

Fibronectin, Rat Plasma MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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      Overview

      Replacement Information
      Description
      OverviewNative fibronectin purified from pooled rat plasma. Effective agent for promoting attachment of cells to commonly-used culture substrates.
      Catalogue Number341668
      Brand Family Calbiochem®
      References
      Product Information
      CAS number86088-83-7
      FormLiquid
      FormulationIn 100 mM NaCl, 20 mM sodium phosphate buffer, pH 7.3, preservative and reductant-free.
      Quality LevelMQ100
      Applications
      Biological Information
      Puritysingle major band by SDS-PAGE
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      ContaminantsNone detected by IEP using anti-fibronectin and anti-rat serum
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C). Be VERY careful during the thawing process. Place the vial in a 37°C water bath and leave it there until it is completely thawed. DO NOT DISTURB OR REMOVE THE VIAL AT ANY TIME DURING THE THAWING PERIOD. If the vial is disturbed or removed prior to complete thawing, the product will gel and be unusable. DO NOT VORTEX. DO NOT SHAKE AFTER THAWING. Mix very gently after thawing.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      341668 0

      Documentation

      Fibronectin, Rat Plasma MSDS

      Title

      Safety Data Sheet (SDS) 

      Fibronectin, Rat Plasma Certificates of Analysis

      TitleLot Number
      341668
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision02-May-2008 RFH
      DescriptionNative fibronectin purified from pooled rat plasma. Effective agent for promoting attachment of cells to commonly-used culture substrates.
      FormLiquid
      FormulationIn 100 mM NaCl, 20 mM sodium phosphate buffer, pH 7.3, preservative and reductant-free.
      Concentration Label Please refer to vial label for lot-specific concentration
      Recommended reaction conditionsProcedure for Coating of Tissue Culture Plates with Fibronectin This procedure is based on the use of 21 cm2 dishes (Corning 25010, Costar 3060, Falcon 3002, or an equivalent 60 mm dish) and the 1 mg package size of fibronectin. One mg of fibronectin is sufficient to coat 10 dishes at 5 µg/cm2. 1. After thawing the fibronectin solution as indicated above, bring the solution to a final volume of 20 ml with sterile serum-free medium that has been preheated to 37°C. This yields a fibronectin solution of 50 µg/ml. 2. Add 2 ml of this solution to each of 10 tissue culture plates and swirl gently to completely coat the entire growth surface. 3. Allow dishes to remain at room temperature for ~45 min to permit binding of the fibronectin to the growth surfaces. 4. Tilt each dish and remove the fibronectin solution using a sterile pipette. Do not permit the pipette tip to disturb the growth surface. 5. Add cell suspension in medium directly to the fibronectin-coated dishes. This procedure is meant to serve as a guideline and optimal may require optimization.
      CAS number86088-83-7
      Puritysingle major band by SDS-PAGE
      ContaminantsNone detected by IEP using anti-fibronectin and anti-rat serum
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C). Be VERY careful during the thawing process. Place the vial in a 37°C water bath and leave it there until it is completely thawed. DO NOT DISTURB OR REMOVE THE VIAL AT ANY TIME DURING THE THAWING PERIOD. If the vial is disturbed or removed prior to complete thawing, the product will gel and be unusable. DO NOT VORTEX. DO NOT SHAKE AFTER THAWING. Mix very gently after thawing.
      Toxicity Standard Handling
      Merck USA index14, 4075