AAMT004 Sigma-AldrichMitochondrial Complex IV (Human) Activity Assay Kit

This product has been discontinued.

Product Description

The Mitochondrial Complex IV (Human) Activity Assay Kit is used to determine the activity of the cytochrome c oxidase enzyme (EC 1.9.3.1) in a human sample. Complex IV is immunocaptured within the wells and activity is determined colorimetrical by following the oxidation of reduced cytochrome c as an absorbance decrease at 550 nm. The overall reaction is as follows:

10 ml 	Buffer, (72571-10ML)
1 ml	Detergent, (72572-1ML)
1 ml	Reduced Cytochrome C, (72573-1ML)
1 ea	Microplate, (72574-1EA)

*This kit contains sufficient matirials for 96 measurements (one 96-well microplate).

Storage: Store the Buffer, Detergent, and Microplate at 4°C. Store the Reduced Cytochrome C at –20°C. For multiple experiments, Reduced Cytochrome C may be aliquoted and stored at –20°C or preferably at –80°C.

This kit contains the necessary buffer, detergent for sample preparation, and substrate (reduced cytochrome c) for the reaction. The kit also contains a 96-well microplate with a monoclonal antibody pre-bound to the wells of the microplate. This plate can be broken into 12 separate 8-well strips for convenience; therefore the plate can be used for up to 12 separate experiments.