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CC324-1 PhotoGel®-LAP, Methacrylated Gelatin Hydrogel Kit

Photocrosslinked gelatin hydrogel used for 3D cell culture and as a bioink for bioprinting (CC324 Kit Component 1)

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CC324-1
1 Kit  
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      Overview

      Replacement Information
      Description
      Catalogue NumberCC324-1
      DescriptionPhotoGel®-LAP, Methacrylated Gelatin Hydrogel Kit
      Overview3D cell culture, including bioprinting, allows for the creation of more physiological cell models by allowing cells to simultaneously interact with integrins on all cell surfaces, resulting in the activation of specific signaling pathways not activated in traditional 2D cell culture methods. Hydrogels are water swollen polymers that allow for the culture of cells in 3-dimensions and can have profound effects on cellular development, differentiation, migration, and function. New areas of tissue engineering such as 3D bioprinting, have utilized UV photocrosslinked methacrylated hydrogel biomaterials (PEGMA, GelMA, HAMA and ColMA etc.) to encapsulate cells to make printable bioinks.

      The PhotoGel®-LAP, Methacrylated Gelatin Hydrogel Kit (CC324: CC324-1, CC324-2) is based upon purified porcine gelatin methacrylate (GelMA), which when photocrosslinked provides a native-like 3D environment for cells. Gelatin derived from denatured collagen retains many natural cell binding motifs such as RGD and MMP sites. In addition to porcine gelatin methacrylate, the kit includes the photoinitiator lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP) for users to easily fine tune their photocrosslinking experiments (i.e. altering hydrogel stiffness or gelling speeds). Gelatin methacrylate is produced from porcine, type A, 300 bloom gelatin. Gelatin macromers containing primary amino groups were reacted with methacrylic anhydride (MA) to add methacrylate pendant groups. The gelatin methacrylate achieves a degree of substitution of >70% for maximum crosslinking and range of stiffness.

      References

      1) Mikos, A, et al. A high-throughput approach to compare the biocompatibility of candidate bioink formulations. Bioprinting Volume 17, March 2020.

      PhotoGel® is a registered trademark of Advanced BioMatrix, Inc.
      Alternate Names
      • Gelatin Methacrylate
      • Gelatin Methacryloyl
      • GelMA
      • Gelatin Hydrogel
      • Gelatin Bioink
      References
      Product Information
      Components
      • CC324-1 (Store at 2-8°C): LAP Photoinitiator, 1 X 100 mg (CS226439).
      • Note: The complete PhotoGel®-LAP, Methacrylated Gelatin Hydrogel Kit (CC324) consists of CC324-1 and CC324-2 sold seperately.
      Applications
      ApplicationPhotocrosslinked gelatin hydrogel used for 3D cell culture and as a bioink for bioprinting (CC324 Kit Component 1)
      Key Applications
      • Cell Culture
      Application NotesPhotocrosslinking is initiated using a 405 nm blue light source.
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceAppearance: Lyophilized powder
      Gelatin Content: ≥ 1 gram
      Sterility (USP modified): No Growth
      pH: 6.0-8.0
      Grafting Efficiency: ≥ 75%
      Cell Compatibility: > 70% Cell Viability
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size1 Kit
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      CC324-1 04061842348138