ECM220 Sigma-AldrichQCM Laminin Migration Assay (24-well, colorimetric)
This QCM Laminin Migration Assay incorporates a 5 um porous membrane, 24-well plate Boyden chamber & mouse laminin, a major extracellular matrix (ECM) protein in basement membrane, to examine cell migration dependent upon the presence of laminin.
More>> This QCM Laminin Migration Assay incorporates a 5 um porous membrane, 24-well plate Boyden chamber & mouse laminin, a major extracellular matrix (ECM) protein in basement membrane, to examine cell migration dependent upon the presence of laminin. Less<<Recommended Products
Overview
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Key Spec Table
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Colorimetric Fluorescent |
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Catalogue Number | ECM220 |
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Description | QCM Laminin Migration Assay (24-well, colorimetric) |
Overview | Also available: Cell Comb™ Scratch Assay! Get biochemical data from a scratch assay! Click Here Introduction Cell migration is a fundamental function of normal cellular processes, including embryonic development, angiogenesis, wound healing, immune response, and inflammation (1, 2). Cell migration may be evaluated using several different methods; the most widely accepted being the Boyden Chamber assay. The Boyden Chamber system uses a two-chamber plate model in which a porous membrane provides an interface between two chambers. Cells are seeded in the upper chamber and chemoattractants placed in the lower chamber. Cells in the upper chamber migrate toward the chemoattractants by passing through the porous membrane to the lower chamber. Migratory cells are stained and quantified. Most cells are sized from 30 to 50 µm can migrate through 3 to 10 µm pore. |
Materials Required but Not Delivered | 1. Precision pipettes, sufficient for aliquoting appropriate volume of cells and reagents. 2. Harvesting buffer: EDTA or trypsin-based cell detachment buffer, or other cell detachment formulations as optimized by individual investigators. Millipore’s ready-to-use non-mammalian detachment solution, Accutase (Cat. No. SCR005) is recommended. Note: Trypsin-based cell detachment buffer may be required for strongly adherent cell lines, but can strip cell surface proteins. Allow sufficient time for cell receptor recovery. 3. Tissue culture growth medium appropriate for subject cells. 4. Quenching Buffer: Serum-free medium such as DMEM or RPMI-1640, containing 5% BSA Note: Quenching Buffer must contain sufficient divalent cations (Mg 2+ or Ca 2+) to quench any EDTA present in the Harvesting Buffer. 5. Chemoattractants (eg. 10% FBS) or pharmacological agents for addition to culture medium, if screening is desired. 6. Sterile PBS (Cat. No. BSS-1005-B) or HBSS to wash cells. 7. Distilled water. 8. Low speed centrifuge and tubes for cell harvesting. 9. CO2 incubator appropriate for subject cells. 10. Hemocytometer or other means of counting cells. 11. Trypan blue or equivalent viability stain. 12. Spectrophotometer (microplate reader with 540-570 nm detection capability). 13. Sterile cell culture hood. 14. (Optional) Graduated ocular (calibrated), or automated method for counting stained cells on a membrane. |
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Components |
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Detection method | Colorimetric Fluorescent |
Quality Level | MQ100 |
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Material Size | 1 kit |
Material Package | Sufficient for 24 samples |
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Catalogue Number | GTIN |
ECM220 | 04053252014635 |
Documentation
QCM Laminin Migration Assay (24-well, colorimetric) MSDS
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References
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Wound healing--aiming for perfect skin regeneration. Martin, P Science, 276: 75-81 (1997) 1997 Show Abstract | 9082989 |
Transient functional expression of alphaVbeta 3 on vascular cells during wound repair. Clark, R A, et al. Am. J. Pathol., 148: 1407-21 (1996) 1996 Show Abstract | 8623913 |
User Guides
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QCM™ Laminin Migration Assay |