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SAE176-100UG SAvPhire Monomeric Streptavidin Alpha Fluor™ 488

SAvPhire Monomeric Streptavidin Alpha Fluor 488, Cat. No. SAE176, is a high-affinity recombinant variant of SA expressed in E. coli that minimizes the risk of aggregation, precipitation, and crosslinking in pre-incubation with biotinylated molecules.

SAE176-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberSAE176-100UG
      DescriptionSAvPhire Monomeric Streptavidin Alpha Fluor™ 488
      Alternate Names
      • SAvPphire-Alpha™488
      • mSA-Alpha™488
      Background InformationStreptavidin, a tetrameric protein, has been extensively used in purification and detection of biomolecules. The strong streptavidin-biotin bond can highly suited for attaching various biomolecules to one another or onto a solid support. The streptavidin-biotin complex displays high resistance to organic solvents, denaturants, detergents, and proteolytic enzymes. It can also withstand extremes of temperature and pH. However, one challenge faced by researchers using the streptavidin-biotin system is the target aggregation, which results from multivalent binding. It can cause cross-linking that can interfere with observations at the molecular level. These challenges can be overcome by use of monomeric streptavidin (mSA) that displays structural and binding properties that are suitable for multiple applications based on its small size and monovalent biotin binding. The monomeric form allows for association with biotinylated ligands without inducing aggregation that are observed with multivalent interactions. Monomeric form can also be genetically fused to another protein to form a biotin-binding tag. SAvPhire Monomeric Streptavidin is a is a high-affinity recombinant variant of streptavidin that minimizes the risk of aggregation, precipitation, and crosslinking in pre-incubation steps with biotinylated molecules and offers easier access to the desired targets. Unlike other non-engineered monomeric forms, SAvPhire Monomeric Streptavidin displays highly desirable dissociation kinetics (koff = 63 x 10-3 min-1 for non-engineered monomeric form and 2.1 x 10-3 min-1 for SAvPhire Monomeric Streptavidin) and longer t1/2 (11 min vs 329 min). It is suitable for multiple immunodetection applications. (Ref.: Kroestch, A., et al., (2018). Appl. Microbiol. Biotechnol., 102(23); 10079-10089).
      References
      Product Information
      FormatHRP
      PresentationSAvPhire Monomeric Streptavidin Alpha Fluor™ 488 lyophilized from PBS containing D-Mannitol and Sucrose without azide.
      Quality LevelMQ200
      Applications
      ApplicationSAvPhire Monomeric Streptavidin Alpha Fluor 488, Cat. No. SAE176, is a high-affinity recombinant variant of SA expressed in E. coli that minimizes the risk of aggregation, precipitation, and crosslinking in pre-incubation with biotinylated molecules.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      • Flow Cytometry
      Application NotesFluorescent Western Blotting Analysis: GFAP was detected in mouse brain tissue lysate probed with a 1:1 mixture of Anti-GFAP-Biotin conjugate (1:1,000 dilution) and SAvPhire Monomeric Streptavidin Alpha Fluor 488.

      Flow Cytometry Analysis: CD4-Biotin was detected in untreated Human peripheral blood mononuclear cells (PBMC) stained with a premixed solution of Anti-CD-4 biotin, clone SK3 and Streptavidin Alpha Fluor 488.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      EpitopeUnknown
      ConcentrationUpon reconstitution in 100 μL of distilled water the concentration will be 1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostE. coli
      SpecificitySAvPhire Monomeric Streptavidin expressed in E. coli and conjugated with Alpha Fluor 488 that is suitable for immunodetection of biotinylated proteins.
      Species Reactivity
      • All
      Species Reactivity NotePredicted to react with all species.
      Antibody TypeMonoclonal Antibody
      UniProt Number
      Molecular Weight15.5 kDa for SAvPhire Monomeric Streptavidin (with an N-terminal His-tag and a C-terminal FLAG® tag).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in A431 cells.



      Immunofluorescent analysis of A431 cells performed using a 1:1 mixture of biotinylated Anti-EGFR (1:1,000 dilution) and SAvPhire Monomeric Streptavidin Alpha Fluor 488 detected EGFR.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      SAE176-100UG 04065265086042