Millipore Sigma Vibrant Logo

PF017 TGF-β2, Human, Recombinant, E. coli

TGF-β2, Human, Recombinant, E. coli MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Transforming Growth Factor-β2

PF017
  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
—
Request Pricing
—
Limited Availability Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

       

      Contact Customer Service

      Overview

      Replacement Information

      Key Spec Table

      Purity
      ≥95% by SDS-PAGE
      Description
      Overview

      This product has been discontinued.





      Full length, recombinant, human TGF-β2 expressed in E. coli. Reactive in many cell types.
      Catalogue NumberPF017
      Brand Family Calbiochem®
      SynonymsTransforming Growth Factor-β2
      References
      ReferencesTsang, M., et al. 1995. Cytokine 7 389.
      Sonis, S.T., et al. 1994. Can. Res. 54, 1135.
      Burton, P.B.J., et al. 1993. Int. J. Expl. Pathol. 74, 87.
      Kondaiah, P., et al. 1990. J. Biol. Chem. 265, 1089.
      Massague, J. 1990. Cell Biol. 6, 597.
      Roberts, A.B. and Sporn, M.B. 1990. In: Peptide Growth Factors and Their Receptors, ed. M. Sporn, A.B. Roberts. Heidelberg: Springer-Verlag.
      Madisen, L., et al. 1988. DNA 7, 1.
      ten Dijke, P., et al. Proc. Natl. Acad. Sci. USA 85, 4715.
      Derynck, R., et al. Nucleic Acids Res. 15, 3188.
      Massague, J. 1987. Cell 49, 437.
      Iwata, K.K., et al. 1985. Cancer Res. 45, 2689.
      Product Information
      FormLyophilized
      FormulationLyophilized from sterile filtered 40% acetonitrile, 5% trehalose, 0.1% TFA, 50 µg BSA/µg cytokine.
      PreservativeNone
      Applications
      Biological Information
      Biological activityED₅₀ = 0.1-0.3 ng/ml as measured by the ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells
      Purity≥95% by SDS-PAGE
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution aliquot and freeze (-20°C or -70°C) for long-term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 3 months at -20°C or -70°C or for up to 1 month at 4°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      PF017 0

      Documentation

      TGF-β2, Human, Recombinant, E. coli Certificates of Analysis

      TitleLot Number
      PF017

      References

      Reference overview
      Tsang, M., et al. 1995. Cytokine 7 389.
      Sonis, S.T., et al. 1994. Can. Res. 54, 1135.
      Burton, P.B.J., et al. 1993. Int. J. Expl. Pathol. 74, 87.
      Kondaiah, P., et al. 1990. J. Biol. Chem. 265, 1089.
      Massague, J. 1990. Cell Biol. 6, 597.
      Roberts, A.B. and Sporn, M.B. 1990. In: Peptide Growth Factors and Their Receptors, ed. M. Sporn, A.B. Roberts. Heidelberg: Springer-Verlag.
      Madisen, L., et al. 1988. DNA 7, 1.
      ten Dijke, P., et al. Proc. Natl. Acad. Sci. USA 85, 4715.
      Derynck, R., et al. Nucleic Acids Res. 15, 3188.
      Massague, J. 1987. Cell 49, 437.
      Iwata, K.K., et al. 1985. Cancer Res. 45, 2689.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision16-September-2008 RFH
      SynonymsTransforming Growth Factor-β2
      DescriptionFull length, recombinant, human TGF-β2 expressed as a chimeric protein (112 amino acids) containing the human TGF-β1 signal and latency associated peptide sequences with the mature TGF-β2 sequence. For use in growth inhibition assays and other assays designed to study cellular responses and receptor interactions involving TGF-β2. Expressed in NSO mouse myeloma cells and purified from conditioned medium. The mature protein is disulfide linked and has a predicted molecular weight of ~25 kDa.

      TGF β is prototypic of a superfamily of proteins with diverse activities. The TGF β proteins and other members of the family show growth, differentiation, and morphogenic activity in a wide range of biological systems being especially involved in many processes of tissue development and repair. Structurally, the TGF β1, β2, and β3 proteins are homodimers composed of disulfide linked chains 112 amino acids in length. Composition of TGF β proteins from different species is highly conserved at the DNA level as well as at the protein level showing in general >85% identity within a subtype and 64-82% identity between subtypes. TGF β1 and β2 were first identified on the basis of their biological activity and subsequently cloned from expression libraries. Human TGF β3 was identified first at the cDNA level and of the three major isoforms, appears to be the most biologically active. The expression of TGF β3 at various stages of morphogenesis and at different sites suggests an important role in the developmental process. Product should be titrated for optimal use in individual systems.
      FormLyophilized
      FormulationLyophilized from sterile filtered 40% acetonitrile, 5% trehalose, 0.1% TFA, 50 µg BSA/µg cytokine.
      Recommended reaction conditionsLyophilized protein must be reconstituted with 50 µl of 10 mM HCl/10% ethanol using siliconized, sterile pipet tips. Working dilutions should be made in sterile medium containing 1-10% serum or 0.1-1% BSA and used immediately, do not store working solutions.
      Purity≥95% by SDS-PAGE
      Biological activityED₅₀ = 0.1-0.3 ng/ml as measured by the ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells
      SolubilityReconstitute to a final concentration of ≥1 µg/ml in sterile 4 mM HCl containing ≥0.1% HSA or BSA.
      PreservativeNone
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution aliquot and freeze (-20°C or -70°C) for long-term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 3 months at -20°C or -70°C or for up to 1 month at 4°C.
      Toxicity Standard Handling
      ReferencesTsang, M., et al. 1995. Cytokine 7 389.
      Sonis, S.T., et al. 1994. Can. Res. 54, 1135.
      Burton, P.B.J., et al. 1993. Int. J. Expl. Pathol. 74, 87.
      Kondaiah, P., et al. 1990. J. Biol. Chem. 265, 1089.
      Massague, J. 1990. Cell Biol. 6, 597.
      Roberts, A.B. and Sporn, M.B. 1990. In: Peptide Growth Factors and Their Receptors, ed. M. Sporn, A.B. Roberts. Heidelberg: Springer-Verlag.
      Madisen, L., et al. 1988. DNA 7, 1.
      ten Dijke, P., et al. Proc. Natl. Acad. Sci. USA 85, 4715.
      Derynck, R., et al. Nucleic Acids Res. 15, 3188.
      Massague, J. 1987. Cell 49, 437.
      Iwata, K.K., et al. 1985. Cancer Res. 45, 2689.