Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
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Select A Species, Panel Type, Kit or Sample Type
To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
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96-Well Plate
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Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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Filtering Orange Juice: Millex® HPF Filters (PVDF) Can Process Higher Volumes Than Other Filters Without Clogging.
Comparison of Millex® HPF syringe filters with PVDF membrane compared to Competitor W syringe filters with PVDF membrane and a prefilter. 10% solutions (v/v) of of orange juice (three different brands) were filtered through these syringe filters and volume at which the filter was clogged was recorded.
Pharmaceutical Product Analysis: Drug Binding Characteristics of Millex® HPF Filters (PVDF and Nylon)
Since Millex® HPF filters contain glass fiber prefilters, they tend to bind the analyte of interest, as seen here with filtration of ranitidine solution through various Millex® syringe filters. All the Millex® HPF filters (PVDF and nylon) showed 15 – 25% drug binding for the first mL of sample filtered. But when the third mL fraction was collected, quantitative drug recovery was obtained. For Millex® syringe filters containing no prefilter (PVDF 0.45), no drug binding was observed even for the first mL of filtrate.
The bottom line: for easy-to-filter samples, always use a membrane-only syringe filter (without prefilter) to reduce analyte-binding. For hard–to-filter (particle laden / viscous) samples, use Millex® HPF filters and discard 3 -5 mL of filtrate prior to collecting the sample for downstream quantitative analysis.
Drug binding characteristics of Millex® HPF Filters. Prefilter-containing syringe filters (Millex® HPF filters, nylon or PVDF, 0.2 µm or 0.45 µm pore sizes) show greater analyte binding than a membrane-only filter, but all filters showed 100% analyte recovery after filtration of 3-5 mL.
Overall Performance Comparison Among Prefilter-Containing Syringe Filters
Products
Available from Merck?
Volume Throughput*
Extractables**
Analyte Binding
Water
Acetonitrile
Methanol
PVDF 0.45 µm
Yes
+/-
+
+
+
PVDF 0.2 µm
No
NA
NA
NA
NA
NA
Nylon 0.45 µm
Yes
+/-
-
-
-
-
Nylon 0.2 µm
Yes
+/-
-
-
-
-
Hydrophilic PTFE 0.45 µm
Yes
+*
+
+
+
+
Hydrophilic PTFE 0.2 µm
Yes
+*
+
+
+
+
* Volume Throughput compared with Competitor W hydrophobic PTFE syringe filters with prefilter. Volume throughput was measured by filtering 3 different matrices (10% orange juice, 2 mg/ mL protein solution, antihistamine syrup solution) through various prefilter-containing syringe filters.
**Degree to which each device leached extractable impurities was measured by filtering the listed solvents through the various syringe filters and analyzing the filtrate using HPLC-UV analysis at 214 nm.
Millex® HPF Filters (Hydrophilic PTFE): Performance in Filtering Particulate-Laden/Viscous Samples
The ability of Millex® HPF filters with hydrophilic PTFE membrane to process various sample types (20% orange juice in water, v/v; protein shake 2 mg/mL, and Benadryl® antihistamine syrup) was tested by measuring the volume filtered before the filter clogged.
Water Flow Rate Comparison: Millex® HPF Filters vs. Competitor W Filters
The rate of water flow through various Millex® HPF filters was compared with water flow through Competitor W syringe filters that also have built-in prefilteres. For the PTFE/polypropylene syringe filters from Competitor W, they were first wetted by 50% ethanol followed by water rinse prior to flow rate measurement.
Performance Comparison: Millex® HPF Filters (Hydrophilic PTFE) vs. Competitor W Filters (Hydrophobic PTFE)
Volume filtered by Millex® HPF with 0.2 µm Hydrophilic PTFE membrane was measured by filtering the listed solutions until the syringe filter clogged. Volume filtered before clogging was compared to volume filtered by Competitor W filters with 0.2 µm hydrophobic PTFE membrane (also with built-in prefilter). The hydrophobic membrane was wetted with 50% ethanol followed by rinsing with water before the samples were applied.