MAPmates Single Plex Assays

Request Information

MILLIPLEX® MAPmate™ Single Plex Biomarker Assays

Designing your own multiplex assay is easy with MILLIPLEX® MAPmate™ assays. Plex up to 8 individual MAPmate™ assays together using our Cell Signaling Buffer and Detection Kit (48-602MAG) or include them in existing MILLIPLEX® cell signaling kits to enhance the panel or serve as controls within the guidelines provided in the protocols.

Important MAPmate™ Guidelines

Consult the protocol prior to use.
  • All magnetic MAPmate™ assays require the Cell Signaling Buffer & Detection Kit (48-602MAG). This kit contains all necessary reagents except the MAPmate™ assay. Both a filter and flat-bottom plate are included for convenience.
    • MILLIPLEX® Lysis Buffer (Cat.# 43-040)
    • MILLIPLEX® Assay Buffer 2 (Cat.# 43-041)
    • MILLIPLEX® Streptavidin-Phycoerythrin (Cat. # 45-001H)
    • MILLIPLEX® Amplification buffer (Cat.# 43-024A)
    • MILLIPLEX® HeLa Cell Lysate:Unstimulated (Cat. # 47-205)
    • 96-well plate and 2 sealers
    • 96-well filter plate and 2 sealers

Each MAPmate™ includes:
  • Capture Antibody (bead-conjugated)
  • Detection Antibody (biotin-conjugated)
  • Positive Control (stimulated cell lysate) for phosphorylated analytes which can be used to qualify assay performance or, with serial dilutions, to develop a standard curve

3 Easy Steps to Designing a Luminex® Multiplex Signaling Assay

Step 1
Choose the analytes you want to plex together and determine their compatibility. Keep in mind that the following cannot be plexed together:
  • MAPmates™ that require a different assay buffer
  • Phosphospecific and total MAPmate™ pairs (e.g. total GSK3β and GSK3β (Ser9)
  • Pan Tyr and phospho-specific MAPmates™ (e.g. Phsopho-EGFR (pan-Tyr) and HSP27 (Ser78)
  • Phospho- MAPmates™ for a single target (e.g. Akt (Ser473) and Akt (Thr308))
  • GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr

Step 2

Order the MILLIPLEX® Cell Signaling Buffer and Detection Kit (Cat. # 48-602MAG), required to run all MAPmate™ assays

Step 3
Order the MAPmate™ products you have selected.

Find and Purchase Your Favorite Analytes & Kits —
Two Tools to Make Ordering Faster and Easier!

Interactive Product Selector

MILLIPLEX® Analyte Kit Finder

Use the kit finder below to choose the correct assays for your biomarkers of interest, so you can obtain the best quality data the first time.
Please select the species reactivity:

Please select the research area:

Please select your analyte(s) of interest:

Design & Purchase Your Own Kit

Already know your analytes of choice? Start designing and purchasing your own kits now!

Choose Customizable Panels & Premixed Kits – OR – Cell Signaling MAPmates™ Assays.

Design and price your MILLIPLEX® Kits.