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Immobilon®-PSQ Membrane

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Immobilon-P<sup>SQ</sup> membrane prevents blow-through.
Featured Benefit
Immobilon®-PSQ membrane prevents blow-through. A sheet of Immobilon®-PSQ was placed behind a strip of Immobilon®-P and Immobilon®-PSQ to capture proteins that passed through. After the transfer, The backup Immobilon®-PSQ membrane showed that no proteins passed through the Immobilon®-PSQ membrane (lanes 7 and 8).
This membrane has higher protein adsorption and sequencing yields than other membranes.

  • 0.2 µm pore size and a large internal structure
  • Higher protein adsorption and sequencing yields than other membranes
  • Recommended for blotting proteins <20 kDa
  • Prevents blow-through of low molecular weight proteins
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Immobilon®-PSQ membrane prevents blow-through.

Molecular weight standards (lanes 1, 3, 5, 7) and calf liver lysate (lanes 2, 4, 6, 8) were transferred to Immobilon®-P or Immobilon®-PSQ membranes. A sheet of Immobilon®-PSQ transfer membrane was placed behind the primary membranes to capture proteins that passed through (lanes 5 and 6 behind Immobilon®-P; lanes 7 and 8 behind Immobilon®-PSQmembrane).