Centricon® Plus-70 Centrifugal Filter |
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The Centricon® Plus-70 centrifugal filter is designed for rapid processing of aqueous biological solutions in volumes ranging from 15 to 70 mL. Centricon® Plus-70 filters concentrate most 70 mL solutions down to 350 μL in as little as 25 minutes. Samples are typically concentrated in the 50X to 200X range, depending on the sample type and starting sample volume. These units are a convenient alternative to dialysis, lyophilization, precipitation techniques or stirred cells. Centrifuge and Rotor Recommendations
Advantages
- >90% typical recovery
- 50X to 200X concentration
- Low hold-up volume
- Polypropylene housing minimizes binding
- True dead stop prevents spinning to dryness
Applications
- Concentrating and desalting chromatography column eluates
- Concentrating monoclonal antibodies
- Concentrating proteins or viruses from culture supernatants
- Clarifying tissue homogenates and cell lysates
- Buffer exchange or diafiltration
Performance
Spin Time with Respect to Filtrate Volume
Typical Retenate (Concentrate) Recovery
| Membrane NMWL | Protein Solute | Protein Retentate |
|---|---|---|
| 10,000 | Cytochrome c, 12.4 kDa (0.25 mg/mL) | 94 |
| 30,000 | Bovine Serum Albumin, 67 kDa (1 mg/mL) | 96 |
| 100,000 | IgG, 156 kDa (1 mg/mL) | 91 |