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MABC975 Anti-Arylsulfatase B Antibody, clone 10G6.1

Anti-Arylsulfatase B Antibody, clone 10G6.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Glycobiology Gene Symbol: ARSB, MPS6 UniProt Number: P15848
MABC975
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IH(P)MCulture SupernatantMonoclonal Antibody
Description
Catalogue NumberMABC975
DescriptionAnti-Arylsulfatase B Antibody, clone 10G6.1
Alternate Names
  • Arylsulfatase B
  • ASB
  • G4S
  • N-acetylgalactosamine-4-sulfatase
Background InformationArylsulfatase B (EC 3.1.6.12; UniProt P15848; also known as ASB, G4S, N-acetylgalactosamine-4-sulfatase) is encoded by the ARSB (also known as MPS6) gene (Gene ID 411) in human. Arylsulfatases consistute a group of enzymes found in the lysosomes of the liver, pancreas, and kidneys. Arylsulfatase B mediates the breakdown of two glycosaminoglycans (GAGs), dermatan sulfate and chondroitin sulfate. Arylsulfatase B-catalyzed removal of sulfate groups from chondroitin-4-sulfate (C4S) leads to the degradation of C4S. Cellular arylsulfatase B expression is also reported to be found in non-lysosomal compartments, including the plasma membrane. Arylsulfatase B is initially produced with a signal peptide sequence (a.a. 1-36), the removal of which yields the mature enzyme. Arylsulfatase B-deficiency due to ARSB mutations results in an accumulation of GAGs in lysosomes, which in turn can lead to Maroteaux–Lamy syndrome or mucopolysaccharidosis VI (MPS6).
References
Product Information
FormatCulture Supernatant
PresentationMouse monoclonal IgG2aκ antibody in tissue culture supernatant without preservatives.
Quality LevelMQ100
Applications
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Arylsulfatase B in human cerebral cortex and kidney tissues.
Biological Information
ImmunogenGST-tagged recombinant human Arylsulfatase B N-terminal fragment.
EpitopeN-terminal region.
Clone10G6.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityExpected to react with both spliced isoforms of human Arylsulfatase B reported by UniProt (P15848-1 and P15848-2).
IsotypeIgG2aκ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • ARSB
  • MPS6
Purification MethodUnpurified
UniProt Number
Molecular Weight~46 kDa observed. Target bands appear larger than the calculated molecular weights of 56.0 kDa (isoform 1) and 42.3 kDa (isoform 2) due to glycosylation.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Arylsulfatase B in 10 µg of HepG2 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABC975 04055977311792