Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

AB2253 Anti-Doublecortin Antibody

AB2253
50 µL  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
R, MWB, IF, IH(P)GpSerumPolyclonal Antibody
Description
Catalogue NumberAB2253
ReplacesAB5910
DescriptionAnti-Doublecortin Antibody
Alternate Names
  • Doublin
  • Lissencephalin-X
  • Lis-X
Background InformationNeuronal migration protein doublecortin (UniProt: O88809; also known as Doublin, Lissencephalin-X, Lis-X) is encoded by the Dcx (also known as Dcn) gene (Gene ID: 13193) in murine species. Doublecortin is a microtubule-associated protein that is expressed almost exclusively in immature neurons. Hence, it has emerged as an important marker of neurogenesis. It is highly expressed in newly produced cells in the neurogenic zones; the sub ventricular zone along the lateral ventricle, and the sub-granular zone (SGZ) of the dentate gyrus. Doublecortin binds to microtubules in migrating cells and as a result promote the movement of these cells. It is reported to direct neuronal migration by regulating the organization and stability of microtubules. Its phosphorylation by MARK1, MARK2, CDK5, and PKA regulates its ability to bind microtubules. Phosphorylation at serine 265 and serine 297 is reported to occur mainly in the neonatal brain and the level of Doublecortin and its phosphorylation declines significantly by postnatal day 21. Mutations in Dcx gene are reported to cause abnormal migration of neurons during development that disrupt the layering of the cortex, leading to epilepsy, cognitive disability, subcortical band heterotopia. Doublecortin may also be involved in cancer metastasis where unstable interactions between doublecortin and microtubules destabilize cytoskeletal organization leading to disorganized movements of cells that can lead to the uncontrolled migration of cancer cells. (Ref.: Ayanlaja, A.A., et al. (2017). Front. Mol. Neurosci. 10; 199; Graham, ME., et al. (2004). Biochem. J. 381(2); 471-481).
References
Product Information
FormatSerum
HS Code3002 15 90
Control
  • Rat Brain lysate.
PresentationPurified guinea pig polyclonal antibody in buffer containing serum with 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide
Quality LevelMQ100
Applications
ApplicationAnti-Doublecortin, Cat. No. AB2253, is a guinea pig polyclonal antibody that detects Doublecortin and is tested for use in Immunohistochemistry (Paraffin) and Immunofluorescence, and Western Blotting.
Key Applications
  • Western Blotting
  • Immunofluorescence
  • Immunohistochemistry (Paraffin)
Application NotesImmunofluorescence Analysis: A 1:250 dilution from a representative lot detected Doublecortin in Mouse cerebellum tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Doublecortin in Mouse and Rat cerebral cortex tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal region of mouse Doublecortin.
EpitopeC-Terminus
HostGuinea Pig
SpecificityThis guinea pig polyclonal antibody specifically detects Doublecortin. It targets an epitope within 16 amino acids from the C-terminal region.
Species Reactivity
  • Rat
  • Mouse
Species Reactivity NoteMouse, Rat. Predicted to react with Human based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryIn the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The protein encoded by this gene is a cytoplasmic protein which appears to direct neuronal migration by regulating the organization and stability of microtubules. The encoded protein contains two doublecortin domains, which bind microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in this gene are a cause of X-linked lissencephaly. Multiple transcript variants encoding at least three different isoforms have been found for this gene. [provided by RefSeq]
Gene Symbol
  • DBCN
  • DC
  • Doublin
  • LISX
  • Lis-X
  • Lissencephalin-X
  • OTTHUMP00000062892
  • SCLH
  • XLIS
  • doublecortex
  • doublecortin
  • doublin
  • lissencephalin-X
Purification MethodGST Depletion
UniProt Number
UniProt SummaryFUNCTION: Seems to be required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCAMKL1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with LIS-1 of an overlapping, but distinct, signaling pathways that promote neuronal migration.

SUBUNIT STRUCTURE: Interacts with tubulin.

SUBCELLULAR LOCATION: Cytoplasm.

TISSUE SPECIFICITY: Highly expressed in neuronal cells of fetal brain (in the majority of cells of the cortical plate, intermediate zone and ventricular zone), but not expressed in other fetal tissues. In the adult, highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas.

DISEASE: Defects in DCX are the cause of lissencephaly X-linked type 1 (LISX1) [MIM:300067]; also called X-LIS or LIS. LISX1 is a classic lissencephaly characterized by mental retardation and seizures that are more severe in male patients. Affected boys show an abnormally thick cortex with absent or severely reduced gyri. Clinical manifestations include feeding problems, abnormal muscular tone, seizures and severe to profound psychomotor retardation. Female patients display a less severe phenotype referred to as 'doublecortex'. Ref.1 Ref.2 Ref.9 Ref.10 Ref.18 Ref.21

Defects in DCX are the cause of subcortical band heterotopia X-linked (SBHX) [MIM:300067]; also known as double cortex or subcortical laminar heterotopia (SCLH). SBHX is a mild brain malformation of the lissencephaly spectrum. It is characterized by bilateral and symmetric plates or bands of gray matter found in the central white matter between the cortex and cerebral ventricles, cerebral convolutions usually appearing normal. Ref.11 Ref.12 Ref.13 Ref.14 Ref.15 Ref.16 Ref.17 Ref.19

A chromosomal aberration involving DCX is found in lissencephaly. Translocation t(X;2)(q22.3;p25.1).

SEQUENCE SIMILARITY: Contains 2 doublecortin domains.

Molecular WeightTarget molecular weight ~42 kDa observed;40.61 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Rat brain tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Doublecortin in Rat brain tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size50 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AB2253 04053252335495

Documentation

Anti-Doublecortin Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-Doublecortin Antibody Certificates of Analysis

TitleLot Number
Anti-Doublecortin 2477980
Anti-Doublecortin 2455684
Anti-Doublecortin - 2398961 2398961
Anti-Doublecortin - 2428719 2428719
Anti-Doublecortin - 2020458 2020458
Anti-Doublecortin - 2073643 2073643
Anti-Doublecortin - 2196111 2196111
Anti-Doublecortin - 2266805 2266805
Anti-Doublecortin - 2341119 2341119
Anti-Doublecortin - 2517797 2517797

References

Reference overviewPub Med ID
Analysing human neural stem cell ontogeny by consecutive isolation of Notch active neural progenitors.
Edri, R; Yaffe, Y; Ziller, MJ; Mutukula, N; Volkman, R; David, E; Jacob-Hirsch, J; Malcov, H; Levy, C; Rechavi, G; Gat-Viks, I; Meissner, A; Elkabetz, Y
Nature communications  6  6500  2015

Show Abstract
25799239 25799239
Aberrant hippocampal neurogenesis contributes to epilepsy and associated cognitive decline.
Cho, KO; Lybrand, ZR; Ito, N; Brulet, R; Tafacory, F; Zhang, L; Good, L; Ure, K; Kernie, SG; Birnbaum, SG; Scharfman, HE; Eisch, AJ; Hsieh, J
Nature communications  6  6606  2015

Show Abstract
25808087 25808087
Combination treatment with ethyl pyruvate and IGF-I exerts neuroprotective effects against brain injury in a rat model of neonatal hypoxic-ischemic encephalopathy.
Rong, Z; Pan, R; Chang, L; Lee, W
International journal of molecular medicine  36  195-203  2015

Show Abstract
25999282 25999282
Systemic attenuation of the TGF-β pathway by a single drug simultaneously rejuvenates hippocampal neurogenesis and myogenesis in the same old mammal.
Yousef, H; Conboy, MJ; Morgenthaler, A; Schlesinger, C; Bugaj, L; Paliwal, P; Greer, C; Conboy, IM; Schaffer, D
Oncotarget  6  11959-78  2015

Show Abstract
26003168 26003168
Identification of distinct ChAT⁺ neurons and activity-dependent control of postnatal SVZ neurogenesis.
Paez-Gonzalez, P; Asrican, B; Rodriguez, E; Kuo, CT
Nature neuroscience  17  934-42  2014

Show Abstract
24880216 24880216
Analysis of Mll1 deficiency identifies neurogenic transcriptional modules and Brn4 as a factor for direct astrocyte-to-neuron reprogramming.
Potts, MB; Siu, JJ; Price, JD; Salinas, RD; Cho, MJ; Ramos, AD; Hahn, J; Margeta, M; Oldham, MC; Lim, DA
Neurosurgery  75  472-82; discussion 482  2014

Show Abstract
24887289 24887289
Onecut1 is essential for horizontal cell genesis and retinal integrity.
Wu, F; Li, R; Umino, Y; Kaczynski, TJ; Sapkota, D; Li, S; Xiang, M; Fliesler, SJ; Sherry, DM; Gannon, M; Solessio, E; Mu, X
The Journal of neuroscience : the official journal of the Society for Neuroscience  33  13053-65, 13065a  2013

Show Abstract
23926259 23926259
Calorie restriction alleviates the age-related decrease in neural progenitor cell division in the aging brain.
Park, JH; Glass, Z; Sayed, K; Michurina, TV; Lazutkin, A; Mineyeva, O; Velmeshev, D; Ward, WF; Richardson, A; Enikolopov, G
The European journal of neuroscience  37  1987-93  2013

Show Abstract
23773068 23773068
EGF transactivation of Trk receptors regulates the migration of newborn cortical neurons.
Puehringer, D; Orel, N; Lüningschrör, P; Subramanian, N; Herrmann, T; Chao, MV; Sendtner, M
Nature neuroscience  16  407-15  2013

Show Abstract
23416450 23416450
TGF-β superfamily gene expression and induction of the Runx1 transcription factor in adult neurogenic regions after brain injury.
Logan, TT; Villapol, S; Symes, AJ
PloS one  8  e59250  2013

Show Abstract
23555640 23555640