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MAB1096 Anti-Tumor Necrosis Factor-α Antibody, clone 195

MAB1096
200 µg  
Purchase on Sigma-Aldrich

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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HICC, IHC, NEUT, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB1096
Replaces04-1114
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Tumor Necrosis Factor-α Antibody, clone 195
Alternate Names
  • TNF-alpha
References
Product Information
FormatPurified
HS Code3002 15 90
PresentationPurified immunoglobulin. Liquid in 0.01M PBS pH 7.1, 0.15M NaCl containing no preservatives. Sterile filtered.
Quality LevelMQ100
Applications
ApplicationThis Anti-Tumor Necrosis Factor-α Antibody, clone 195 is validated for use in IC, IH, NEUT, WB for the detection of Tumor Necrosis Factor-α.
Key Applications
  • Immunocytochemistry
  • Immunohistochemistry
  • Neutralizing
  • Western Blotting
Application NotesWestern Blot

Immunocytochemistry

Immunohistochemistry

Neutralization: 50 ng of TNF-alpha antibody is required for the neutralization of 1 U TNF-alpha. The TNF-alpha-preparation which is used has a specific activity of 2 x 10(7) U/mg protein [cell lytic assay with L929 cells (mouse transformed fibroblasts) and/or WEHJ 164 cells (mouse fibrosarcoma cells)/actinomycin D].

Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenHuman, recombinant tumor necrosis factor-alpha produced in E. coli.
Clone195
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityThe antibody reacts with human, natural and recombinant TNF-alpha. lt does not react with human TNF-beta (lymphotoxin). lt does not react with TNF-alpha from mouse, rat, canine, porcine, rhesus-monkey or baboon.

SPECIES REACTIVITIES:

Not mouse, rat, canine, porcine, baboon or rhesus-monkey.
IsotypeIgG3
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
Gene Symbol
  • TNF
  • OTTHUMP00000037669
  • TNFA
  • TNFSF2
  • cachectin
  • TNF-a
  • Cachectin
  • DIF
  • TNF-alpha
Non-Reactive Species
  • Canine
  • Mouse
  • Monkey
  • Pig
  • Rat
  • Baboon
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P01375 # Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
SIZE: 233 amino acids; 25644 Da
SUBUNIT: Homotrimer.
SUBCELLULAR LOCATION: Cell membrane; Single-pass type II membrane protein. & Tumor necrosis factor, soluble form: Secreted.
PTM: The soluble form derives from the membrane form by proteolytic processing. & The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
DISEASE: SwissProt: P01375 # Cachexia accompanies a variety of diseases, including cancer and infection, and is characterized by general ill health and malnutrition. & Genetic variations in TNF are associated with susceptibility to psoriatic arthritis [MIM:607507]. Psoriasis is a chronic inflammatory dermatosis that affects approximately 2% of the population. It is characterized by red, scaly skin lesions that are usually found on the scalp, elbows, and knees, and may be associated with severe arthritis. Psoriatic arthritis has been defined as an inflammatory arthritis usually without any rheumatoid factor in serum (seronegative arthritis) associated with psoriasis. & Genetic variations in TNF are associated with susceptibility to hepatitis B virus infection (HBV infection) [MIM:610424]. Approximately one third of all cases of cirrhosis and half of all cases of hepatocellular carcinoma can be attributed to chronic HBV infection. HBV infection may result in subclinical or asymptomatic infection, acute self-limited hepatitis, or fulminant hepatitis requiring liver transplantation.
SIMILARITY: SwissProt: P01375 ## Belongs to the tumor necrosis factor family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain frozen at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. Freeze only once.
Packaging Information
Material Size200 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB1096 04053252267505

Documentation

Anti-Tumor Necrosis Factor-α Antibody, clone 195 SDS

Title

Safety Data Sheet (SDS) 

Anti-Tumor Necrosis Factor-α Antibody, clone 195 Certificates of Analysis

TitleLot Number
Anti-Tumor Necrosis Factor-alpha, clone 195 MONOCLONAL ANTIBODY Q2916206
MOUSE ANTI-TUMOR NECROSIS FACTOR-#945; MONOCLONAL ANTIBODY - 2121752 2121752
MOUSE ANTI-TUMOR NECROSIS FACTOR-#945; MONOCLONAL ANTIBODY - 2136612 2136612
MOUSE ANTI-TUMOR NECROSIS FACTOR-a MONOCLONAL ANTIBODY 2935464
MOUSE ANTI-TUMOR NECROSIS FACTOR-a MONOCLONAL ANTIBODY 2952659
MOUSE ANTI-TUMOR NECROSIS FACTOR-a MONOCLONAL ANTIBODY - 2110486 2110486
MOUSE ANTI-TUMOR NECROSIS FACTOR-a MONOCLONAL ANTIBODY - 2299548 2299548
MOUSE ANTI-TUMOR NECROSIS FACTOR-a MONOCLONAL ANTIBODY - 2332529 2332529
MOUSE ANTI-TUMOR NECROSIS FACTOR-α - 3488920 3488920
MOUSE ANTI-TUMOR NECROSIS FACTOR-α - 3698113 3698113

References

Reference overviewPub Med ID
Acute bronchial obstruction in sheep: histopathology and gland cytokine expression.
Robert A Cox, Ann S Burke, Gloria Oliveras, Perenlei Enkhbaatar, Lillian D Traber, Joseph B Zwischenberger, Marc G Jeschke, Frank C Schmalstieg, David N Herndon, Daniel L Traber, Hal K Hawkins
Experimental lung research  31  819-37  2005

Show Abstract
16684715 16684715
Monocyte-mediated drug-dependent cellular cytotoxicity: effects on different WEHI 164 target cell lines.
Austgulen, R, et al.
Cancer Immunol. Immunother., 22: 176-80 (1986)  1986

Show Abstract
3731202 3731202
Human lymphotoxin.
Aggarwal, B B
Meth. Enzymol., 116: 441-8 (1985)  1985

3878923 3878923
Human tumor necrosis factor.
Aggarwal, B B and Kohr, W J
Meth. Enzymol., 116: 448-56 (1985)  1985

4088089 4088089

Data Sheet

Title
MOUSE ANTI-TUMOR NECROSIS FACTOR-α

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Product Families

Categories

Life Science Research > Antibodies and Assays > Primary Antibodies