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SCT070 BioTracker NIM-7 Lipids and Lysosomes Live Cell Dye

Live cell imaging fluorescent probe for simultaneous dual-color tracking of lipid droplets and lysosomes

SCT070
1 mg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberSCT070
DescriptionBioTracker NIM-7 Lipids and Lysosomes Live Cell Dye
OverviewLipid droplets (LDs) and lysosomes are specialized subunits within cells. LDs are the most hydrophobic sub-structures within cells and serve to store lipids for energy generation and membrane synthesis. Lysosomes are typically acidic organelles (pH 4.5–5.0) that play a critical role in the digestion and clearance of endocytosed and endogenous intracellular materials.

BioTracker NIM-7 Lipids and Lysosomes Live Cell Dye is a naphthalimide-based fluorescent probe that allows lipid droplets and lysosomes to be labelled simultaneously and visualized separately using different excitation and detection channels. Lipid droplets and lysosomes can be visualized in the yellow and red fluorescence regions, respectively. The dye was found to have low cytotoxicity in multiple cell lines after 24 hour incubation. The dye also high photostability and can maintain 90% of original fluorescence intensity after 15 min of irradiation.

Spectral properties:
Lipid droplets: Excitation: 488 nm. Emission: 530-570 nm.
Lysosomes: Excitation: 561 nm. Emission: 640-680 nm.

References:
Zheng X et al. Simultaneous dual-color tracking lipid droplets and lysosomes dynamics using a fluorescent probe. Chem. Sci. 2019, 10, 2342.
Alternate Names
  • Live cell imaging probe
  • Lipid probe
  • Lysosomes probe
References
Product Information
Detection methodFluorescence
PresentationLyophilized
Applications
ApplicationLive cell imaging fluorescent probe for simultaneous dual-color tracking of lipid droplets and lysosomes
Key Applications
  • Cell Based Assays
Application NotesLive cell fluorescent imaging
Biological Information
Molecular Weight610.57
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssurancePurity: ≥ 98% confirmed by HPLC. Structure confirmed by HNMR, LC-MS and elemental analysis
Molar Mass: 610.57 g/mol
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore BioTracker NIM-7 Lipids and Lysosomes Live Cell Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Packaging Information
Material Size1 mg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
SCT070 04061841441069