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CYT306 ChemiKine Brain Derived Neurotrophic Factor, Sandwich ELISA

1 kit  
Purchase on Sigma-Aldrich


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Key Spec Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
BDNF H, RELISAChromogenic
Catalogue NumberCYT306
Brand Family Chemicon®
Trade Name
  • ChemiKine
  • Chemicon
DescriptionChemiKine Brain Derived Neurotrophic Factor, Sandwich ELISA
OverviewChemiKine™ Brain Derived Neurotrophic Factor (BDNF) kit is a sandwich enzyme immunoassay (EIA), which measures BDNF. The kit will measure BDNF from human and rat.

Test Principle:

With the ChemiKine BDNF assay system, rabbit polyclonal antibodies generated against human BDNF are coated onto a microplate and are used to capture BDNF from a sample. BDNF specific, biotin conjugated, mouse monoclonal antibodies detect the captured BDNF. After addition of streptavidin-enzyme, substrate and stop solution the amount of BDNF is determined. The standard curve demonstrates a direct relationship between Optical Density (OD) and BDNF concentration: i.e., the higher the OD the higher the BDNF concentration in the sample.


ChemiKine BDNF kit is designed to measure the amount of BDNF in cell culture supernatants, tissue homogenates and biological fluid (serum, plasma, and serum-free) samples from human and rat. There are enough reagents included in this kit for two 96-well immuno-assay plates. Running duplicate wells for samples and standards is recommended.

For Research Use Only. Not for use in diagnostic procedures.
Alternate Names
  • BDNF
Materials Required but Not Delivered1. Multi-channel or repeating pipettes

2. Plate shaker (optional)

3. Pipettors & tips capable of accurately measuring 10-1000 mL

4. Graduated serological pipettes

5. 96-well microplate Reader with 450 nm filter

6. Graph paper for manual plotting of data

7. Polystyrene test tubes for standard and sample dilutions

8. Mechanical vortex

9. One 1 or 2 liter container
Product Information
  • ChemiKine BDNF ELISA Plate: (Part No. 60238) Two Pre-coated with Rabbit anti-Human BDNF Polyclonal Antibody 96-Well Immunoplates sealed in foil pouches.
  • Wash Buffer Concentrate: (Part No. 60245) One 100 mL (10X) bottle of Concentrate.
  • Standard/Sample Diluent: (Part No. 60240) One 60 mL bottle (Ready to Use).
  • BDNF Standard (Recombinant Human): (Part No. 60237) Two vials (Lyophilized).
  • Biotinylated Mouse anti-Human BDNF Monoclonal Antibody: (Part No. 60583) One 25 μL vial.
  • Streptavidin-Enzyme conjugate: (Part No. 60582) One 50 mL vial of HRP conjugated Streptavidin.
  • TMB/E Solution: (Part No. 60096) Two 10 mL bottles of a Ready to Use solution of 3,3',5,5'-tetramethylbenzidine in a proprietary buffer with enhancer.
  • Stop Solution (Part No. 60260): One 22 mL bottle of an HCl solution.
Detection methodChromogenic
HS Code3002 15 90
Quality LevelMQ200
ApplicationThis ChemiKine Brain Derived Neurotrophic Factor, Sandwich ELISA is used to measure & quantify BDNF levels in Neuroscience research.
Key Applications
Biological Information
Species Reactivity
  • Human
  • Rat
Analytes Available
  • BDNF
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer's and Huntington disease patients. This gene may play a role in the regulation of stress response and in the biology of mood disorders. Multiple transcript variants encoding distinct isoforms have been described for this gene, but the full-length nature of only some could be determined.
Gene Symbol
  • BDNF
  • Abrineurin
  • neurotrophin
  • MGC34632
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P23560 # Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
SIZE: 247 amino acids; 27818 Da
SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB.
PTM: The propeptide is N-glycosylated and glycosulfated. & Converted into mature BDNF by plasmin (PLG) (By similarity).
DISEASE: SwissProt: P23560 # Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS) [MIM:209880]; also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases.
SIMILARITY: SwissProt: P23560 ## Belongs to the NGF-beta family.
Physicochemical Information
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • For research use only. Not for use in diagnostic procedures.
Storage and Shipping Information
Storage ConditionsPrecautions:

· Wash Buffer and Sample Buffer contain thimerosal. Thimerosal is highly toxic by inhalation, contact with skin or if swallowed. Thimerosal is a possible mutagen and should be handled accordingly.

· The instructions provided have been designed to optimize the kit's performance. Deviation from the instructions may result in suboptimal performance of the kit and the failure to produce accurate data.
Packaging Information
Material Size1 kit
Transport Information
Supplemental Information
Global Trade Item Number
Catalogue Number GTIN
CYT306 08436037122576


ChemiKine Brain Derived Neurotrophic Factor, Sandwich ELISA SDS


Safety Data Sheet (SDS) 


Reference overviewSpeciesPub Med ID
Decreased levels of brain-derived neurotrophic factor in the remitted state of unipolar depressive disorder.
B J Hasselbalch,U Knorr,B Bennike,S G Hasselbalch,M H Greisen Søndergaard,L Vedel Kessing
Acta psychiatrica Scandinavica  126  2012

Show Abstract
22292883 22292883
BDNF as an effect modifier for gender effects on pain thresholds in healthy subjects.
Luciana Cadore Stefani,Iraci Lucena da Silva Torres,Izabel Cristina Custodio de Souza,Joanna Ripoll Rozisky,Felipe Fregni,Wolnei Caumo
Neuroscience letters  514  2012

Show Abstract
22395087 22395087
Propofol interacts with stimulus intensities of electroconvulsive shock to regulate behavior and hippocampal BDNF in a rat model of depression.
Jie Luo,Su Min,Ke Wei,Junfang Zhang,Yuanyuan Liu
Psychiatry research  198  2012

Show Abstract
22410590 22410590
Various levels of plasma brain-derived neurotrophic factor in patients with tinnitus.
Fumiyuki Goto,Juri Saruta,Sho Kanzaki,Masahiro To,Tomoko Tsutsumi,Keiichi Tsukinoki,Kaoru Ogawa
Neuroscience letters  510  2012

Show Abstract
22281446 22281446
Prolonged therapeutic hypothermia does not adversely impact neuroplasticity after global ischemia in rats.
Gergely Silasi,Ana C Klahr,Mark J Hackett,Angela M Auriat,Helen Nichol,Frederick Colbourne
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism  32  2012

Show Abstract
22434072 22434072
Expression and localization of brain-derived neurotrophic factor (BDNF) mRNA and protein in human submandibular gland.
Juri Saruta,Kazuhiro Fujino,Masahiro To,Keiichi Tsukinoki
Acta histochemica et cytochemica  45  2012

Show Abstract
23012486 23012486
Increase in brain-derived neurotrophic factor in first episode psychotic patients after treatment with atypical antipsychotics.
González-Pintoa A, Mosquera F, Palomino A, Alberich S, Gutiérrez A, Haidar K, Vega P, Barbeito S, Ortiz A, Matute C
Int Clin Psychopharmacol  25  241-5.  2010

Show Abstract
20568658 20568658
Serum MIP-1 alpha level: a biomarker for the follow-up of lentiviral therapy in mucopolysaccharidosis IIIB mice.
Paola Di Natale,Carmela Di Domenico,Daniele Di Napoli
Journal of inherited metabolic disease  33  2010

Show Abstract
20162365 20162365
IFATS collection: The conditioned media of adipose stromal cells protect against hypoxia-ischemia-induced brain damage in neonatal rats.
Xing Wei, Zhimei Du, Liming Zhao, Dongni Feng, Gang Wei, Yongzheng He, Jiangning Tan, Wei-Hui Lee, Harald Hampel, Richard Dodel, Brian H Johnstone, Keith L March, Martin R Farlow, Yansheng Du
Stem cells (Dayton, Ohio)  27  478-88  2009

Show Abstract
19023032 19023032
An analysis of time-dependent changes of neurotrophic factors (BDNF, CNTF) in traumatic facial nerve injury of a nerve-cut and nerve-crush model in rats.
Sertac Yetiser,Erkan Kahraman
Otology & neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology  29  2008

Show Abstract
18494143 18494143


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ChemiKine¿ Brain Derived Neurotrophic Factor (BDNF) Sandwich ELISA Kit