Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on More

100327 Hydrochloric acid in ethanol

Purchase on Sigma-Aldrich


Replacement Information


Catalogue NumberPackaging Qty/Pack
1003271000 Plastic bottle 1 l
1003275000 Plastic container 5 l
Catalogue Number100327
SynonymsHydrogen chloride solution
DescriptionHydrochloric acid in ethanol
OverviewHydrochloric acid in ethanol - for microscopy, is used for human-medical cell diagnosis and serves the purpose of the bacteriological and histological investigation of sample material of human origin. Acid-fast bacteria are difficult to stain because of the high proportion of lipid and wax in their cell walls. The most efficient staining method is the hot staining according to Ziehl-Neelsen. In this method, carbolfuchsin solution is applied to the specimen and heated. This heating process accelerates the rate at which the fuchsin dye is absorbed and thus also that of the formation of the mycolatefuchsin complex in the cell wall. With the Kinyoun method, a modification of the AFB-Color carbol fuchsin solution renders heating unnecessary (cold staining). Once the acid-fast bacteria have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even when they are intensively treated with a decolorizing solution such as e. g. hydrochloric acid in ethanol ( Accordingly, acid-fast bacteria are termed as acid- and alcohol-fast for staining, and are stained red in the microscopic visualization. Correspondingly, all non-acid-fast microorganisms are counterstained with an appropriate dye. In this instruction for use malachite green is used for counterstaining.
Pretreatment of the specimens with Sputofluol® dissolves the bacteria from the surrounding viscid sputum and cell material. It is an IVD and CE registered product and can be applied for diagnostical purposes. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
Product Information
HS Code3824 99 92
Quality LevelMQ400
Applicationfor microscopy
Biological Information
Physicochemical Information
Density0.90 g/cm3 (20 °C)
Flash point21 °C
pH value1.0 (H₂O, 20 °C)
Materials Information
Toxicological Information
Safety Information according to GHS
Hazard Pictogram(s) Hazard Pictogram(s)
Hazard Pictogram(s)
Hazard Statement(s)H225: Highly flammable liquid and vapour.
H290: May be corrosive to metals.
H319: Causes serious eye irritation.
Precautionary Statement(s)P210: Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
P233: Keep container tightly closed.
P234: Keep only in original packaging.
P240: Ground and bond container and receiving equipment.
P241: Use explosion-proof electrical/ ventilating/ lighting equipment.
P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P403 + P233: Store in a well-ventilated place. Keep container tightly closed.
Signal WordDanger
Storage class3 Flammable liquids
WGKWGK 1 slightly hazardous to water
Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.
Safety Information
Categories of dangerflammable
Product Usage Statements
Storage and Shipping Information
StorageStore at +2°C to +30°C.
Packaging Information
Transport Information
Declaration (railroad and road) ADR, RIDUN 2924 , 3 (8), II
Declaration (transport by air) IATA-DGRUN 2924 , 3 (8), II
Declaration (transport by sea) IMDG-CodeUN 2924 , 3 (8), II, Segregation Group: 1 (Acids)
Supplemental Information
Global Trade Item Number
Catalogue Number GTIN
1003271000 04022536004907
1003275000 04022536004921

Related Products & Applications

Accessories (Primary)

Catalogue Number Description
116450 AFB-Color staining kit

Accessories (Secondary)

Catalogue Number Description
100497 AFB-Color modified
109215 Ziehl-Neelsen carbol-fuchsin solution

Related Products By: Application Facete


IVD/OEM Materials & Reagents > Microscopy > Universal Reagents > Auxiliaries for Microscopy
IVD/OEM Materials & Reagents > Microscopy > Bacteriology > Acid-Fast Bacteria Staining