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47-229 MILLIPLEX MAP HeLa Cell Lysate: Lambda Phosphatase - Lysates/Reagents for Multiplex Assays

47-229
100 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Key Applications
Mplex, WB
Description
Catalogue Number47-229
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP HeLa Cell Lysate: Lambda Phosphatase - Lysates/Reagents for Multiplex Assays
OverviewOne vial containing 200 μg of total proteins lyophilized from 100 μL of cell lysate made from HeLa cells that were lysed in MILLIPLEX MAP Lysis Buffer with protease inhibitors and treated with lambda phosphatase (1,000U/mg total protein for 30 min. at 37֯°C).
References
Product Information
Detection methodLuminex xMAP Fluorescent
Quality LevelMQ200
Applications
ApplicationThis MILLIPLEX MAP HeLa Cell Lysate: Lambda Phosphatase, is a control lysate used for multiplex Cell Signaling bead-based kits.
Key Applications
  • Multiplexing
  • Western Blotting
Application NotesHeLa Cell Lysate: Lambda Phosphatase is recommended for use in Western blotting (starting at 20 – 40 μg/well), immunoprecipitation (starting at 40 – 100 μg per sample), and Luminex® bead assays (starting at 2 - 20 μg/well)
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at 2-8°C.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
47-229 04053252518522