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71228 pSTBlue-1 AccepTor™ Vector Giga Kit - Novagen

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Replacement Information


Catalogue NumberPackaging Qty/Pack
71228-3 Plastic ampoule 20 rxn
71228-4 Plastic ampoule 40 rxn

The pSTBlue-1 AccepTor™ Vector Giga Kits and pSTBlue-1 Perfectly Blunt® Giga Cloning Kits feature NovaBlue GigaSingles™ Competent Cells for increased transformation efficiency. AccepTor Vector Kits are designed for the cloning of PCR products generated using thermostable DNA polymerases that leave single 3′-dA nucleotide overhangs. The vector enables direct ligation by virtue of single 3′-dU overhangs that anneal with 3′-dA extensions on PCR products. The dU residues are converted to dT residues in vivo.

The Perfectly Blunt Cloning Kits are designed for the cloning of DNA generated by PCR using any type of DNA polymerase. This allows for the use of high-fidelity proofreading enzymes for amplification, thereby decreasing the probability of generating mutations in the target sequence.

Catalogue Number71228
Brand Family Novagen®
Features and benefits• No restriction enzymes or special primers
• Direct ligation of PCR product with vector
• Compatible with polymerases that leave single 3´-dA overhangs
• Blue/white screening with pSTBlue-1 or pETBlue™-1 vectors
• Simple protocol takes as little as 40 minutes from PCR product to plating transformants
Product Information
DeclarationThis product is covered by U.S. Patent 5,856,144 owned by EMD Chemicals Inc. or its Affiliates.
Quality LevelMQ100
Biological Information
Physicochemical Information
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage ≤ -70°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Global Trade Item Number
Catalogue Number GTIN
71228-3 04055977271133
71228-4 04055977271140

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Catalogue Number Description
70594 Introductory pSTBlue-1 AccepTor™ Vector Kit - Novagen
70595 pSTBlue-1 AccepTor™ Vector Kit - Novagen
70596 pSTBlue-1 AccepTor™ Vector (linearized vector) - Novagen
70597 Introductory pETBlue-1 AccepTor™ Vector Kit - Novagen
70598 pETBlue-1 AccepTor™ Vector Kit - Novagen
70599 pETBlue-1 AccepTor™ Vector (linearized vector) - Novagen

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Life Science Research > Genomic Analysis > DNA Preparation & Cloning > Cloning > Cloning Kits