P5542

Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus

Name: Phospholipase C, Phosphatidylinositol-specific from <I>Bacillus cereus</I>
Brand: SIGMA

lyophilized powder, ≥1,000 units/mg protein (Lowry)

Synonym(s):

1-Phosphatidyl-D-myo-inositol inositol phosphohydrolase, cyclic-phosphate forming, PI-PLC

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All Photos(3)

About This Item

CAS Number:

37288-19-0

UNSPSC Code:

12352204

NACRES:

NA.32

EC Number:

232-638-2

MDL number:

MFCD00131896

EC Number:

4.6.1.13 (BRENDA, IUBMB)

Product Name

Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus, lyophilized powder, ≥1,000 units/mg protein (Lowry)

form

lyophilized powder

specific activity

≥1,000 units/mg protein (Lowry)

foreign activity

Phopholipase C (lecithinase) ≤1 units/mg protein
Sphingomyelinase ≤40 units/mg protein

storage temp.

−20°C

Quality Level

300

Gene Information

Bacillus cereus E33L ... BCZK3513(3026815)

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Analysis Note

Acetylcholinesterase is measured according to Ellman, et al.

Application

Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus has been used to cleave GPI-anchored protein from green fluorescent protein- glycosylphosphatidylinositol (GFP-GPI) from the cell membrane. It has also been used in invasion assay to test the involvement of PLAP in T. cruzi invasion to human epithelial type 2 (HEp-2) cells.

Biochem/physiol Actions

Used for the release of GPI anchored proteins from the membrane.

General description

Phosphatidylinositol-specific phospholipase C (PI-PLC) from Bacillus cereus is a 298 residual protein. It belongs to the class of ubiquitous enzymes. PI-PLC is characterized with a single globular domain and it folds into eight (βα) barrel sheets, out of which only six βα units are found in barrel, lacking helices between strands IV and V and V and VI.

Other Notes

One unit will liberate one unit of acetylcholinesterase per minute from a membrane-bound crude preparation at pH 7.4 at 30 °C (10 minute incubation).

Physical form

Contains phosphate buffer salts, EDTA and stabilizer

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number

0000439663

0000451721

0000119075

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Role of placental alkaline phosphatase in the internalization of trypomatigotes of Trypanosoma cruzi into HEp2 cells.

Sartori MJ

Tropical Medicine & International Health, 8(9), 832-839 (2003)

Crystal structure of the phosphatidylinositol-specific phospholipase C from Bacillus cereus in complex with myo-inositol.

Heinz DW

The Embo Journal, 14(16), 3855-3863 (1995)

The iron chelator, PBT434, modulates transcellular iron trafficking in brain microvascular endothelial cells.

Danielle K Bailey et al.

PloS one, 16(7), e0254794-e0254794 (2021-07-27)

Iron and other transition metals, such as copper and manganese, are essential for supporting brain function, yet over-accumulation is cytotoxic. This over-accumulation of metals, particularly iron, is common to several neurological disorders; these include Alzheimer's disease, Parkinson's disease, Friedrich's ataxia

Imaging of mobile long-lived nanoplatforms in the live cell plasma membrane.

Brameshuber M

The Journal of Biological Chemistry, 285(53), 41765-41771 (2010)

The Drosophila NCAM homolog Fas2 signals independently of adhesion.

Helen Neuert et al.

Development (Cambridge, England), 147(2) (2019-12-22)

The development of tissues and organs requires close interaction of cells. To achieve this, cells express adhesion proteins such as the neural cell adhesion molecule (NCAM) or its Drosophila ortholog Fasciclin 2 (Fas2). Both are members of the Ig-domain superfamily

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Glycosylphosphatidylinisotol-anchored Glycoproteins Overview

Glycosylphosphatidylinisotol- (GPI) anchored proteins have been identified throughout a broad range of eukaryotic species ranging from humans to insects, yeasts, bacteria, and fungi, suggesting that they are an ancient modification.