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Merck

05-1951-AF488

Anti-trimethyl Histone H3 (Lys27) Antibody, clone 18E9.1, Alexa Fluor™ 488 Conjugate

clone 18E9.1, from mouse, ALEXA FLUOR™ 488

Synonym(s):

Histone H3.3, H3F3A, H3.3A, H3F3

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

conjugate

ALEXA FLUOR™ 488

antibody form

purified antibody

antibody product type

primary antibodies

clone

18E9.1, monoclonal

species reactivity

human, mouse

technique(s)

immunocytochemistry: suitable

NCBI accession no.

NP_002098

UniProt accession no.

P84243

shipped in

wet ice

target post-translational modification

trimethylation (Lys27)

Gene Information

human ... H3F3B(3021)

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
17 kDa calculated

Immunogen

Linear peptide corresponding to trimethyl Histone H3 (Lys27).

Application

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected trimethyl Histone H3 (Lys27) in NIH/3T3 cells.
Research Category
Epitope Tags & General Use
Research Sub Category
Epitope Tags
This Anti-trimethyl Histone H3 (Lys27) Antibody, clone 18E9.1, Alexa Fluor™ 488 Conjugate is validated for use in ICC for the detection of trimethyl Histone H3 (Lys27).

Physical form

Protein G Purified
Purified mouse conjugate in buffer containing PBS, 15 mg/ml BSA, and 0.1% Sodium Azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Immunocytochemistry Analysis in HepG2 cells

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected trimethyl Histone H3 (Lys27) in HepG2 cells.

Alexa Fluor™ is a registered trademark of Life Technologies.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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