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Merck

05-806-AF555

Sigma-Aldrich

Anti-phospho-Histone H3 (Ser10) Antibody, clone 3H10, Alexa Fluor™ 555

clone 3H10, from mouse, ALEXA FLUOR™ 555

Synonym(s):

H3S10P, Histone H3 (phospho Ser10), H3 histone, family 3A, H3.3A, H3 histone, family 3B, H3.3B

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

conjugate

ALEXA FLUOR™ 555

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

3H10, monoclonal

species reactivity

human

technique(s)

immunocytochemistry: suitable

isotype

IgG1κ

NCBI accession no.

NM_003493

UniProt accession no.

Q16695

shipped in

wet ice

target post-translational modification

phosphorylation (pSer10)

Gene Information

human ... H3C1(8350)

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci.
~17 kDa observed.

Immunogen

Epitope: H3 pSer10.

Application

Anti-phospho-Histone H3 (Ser10) Antibody, clone 3H10, Alexa Fluor™ 555 is an antibody against phospho-Histone H3 for use in Immunocytochemistry.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected phospho-Histone H3 (Ser10) in HepG2 cells.
The unconjugated antibody (Cat. No. 05-806) is shown to be suitable also for peptide inhibition assay (PIA), flow cytometry, immunocytochemistry, immunofluorescence, multiplex assay, Western blotting applications.

Biochem/physiol Actions

Broad species cross-reactivity expected
Clone 3H10 recoginzes histone N-terminal peptide with Ser10 phosphorylation (pSer10) by bead-based peptide specificity assay. Clone 3H10 does not bind unphosphorylated peptides or peptides with only pThr11 or pSer28 modification.

Physical form

Purified mouse monoclonal IgG1κ antibody conjugate in PBS with 15mg/ml BSA and 0.1 % sodium azide.

Analysis Note

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected phospho-Histone H3 (Ser10) in HeLa cells.

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Global Trade Item Number

SKUGTIN
05-806-AF55504055977311938

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