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Merck

06-519-AF647

Anti-phospho-CREB (Ser133) Antibody, Alexa Fluor™647 conjugate

from rabbit, ALEXA FLUOR™ 647

Synonym(s):

Cyclic AMP-responsive element-binding protein 1 (pSer133), cAMP-responsive element-binding protein 1 (pSer133), CREB-1 (pSer133), CREB-A (pSer133), htCREB (pSer133), CREB-B (pSer119)

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.45
eCl@ss:
32160702

Key Documents

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Product Name

Anti-phospho-CREB (Ser133) Antibody, Alexa Fluor™647 conjugate, from rabbit, ALEXA FLUOR™ 647

biological source

rabbit

conjugate

ALEXA FLUOR™ 647

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, rat, human

species reactivity (predicted by homology)

porcine (based on 100% sequence homology), guinea pig (based on 100% sequence homology), Xenopus (based on 100% sequence homology), canine (based on 100% sequence homology), chicken (based on 100% sequence homology), bovine (based on 100% sequence homology)

technique(s)

immunofluorescence: suitable

isotype

IgG1

NCBI accession no.

NP_604391

UniProt accession no.

P16220

shipped in

wet ice

target post-translational modification

phosphorylation (pSer133)

Quality Level

100

Gene Information

human ... CREB1(1385)

Analysis Note

Evaluated by Immunofluorescence in rat brain tissue.

Immunofluorescence Analysis: A 1:50 dilution of this antibody detected phospho-CREB (Ser133) in rat brain tissue.

Application

Anti-phospho-CREB (Ser133) Antibody, Alexa Fluor™647 conjugate is an antibody against phospho-CREB for use in Immunofluorescence.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
The unconjugated antibody (Cat. No. 06-519) is shown to be suitable also for Electrophoretic Mobility Shift Assay (EMSA), immunohistochemistry, and Western blotting applications.

Biochem/physiol Actions

Recognizes phosphorylated CREB-A (pSer133), htCREB (pSer133), and CREB-B (pSer119) spliced isoforms reported by (UniProt P16220). May also recognize phosphorylated CREM and ATF-1 that contain homologous sequence to that of the immungen peptide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Cyclic AMP-responsive element-binding protein 1 (UniProt P16220; also known as cAMP-responsive element-binding protein 1, CREB-1) is encoded by the CREB1 gene (Gene ID 1385) in human. CREB is a transcription factor that binds the cAMP response elements (CRE) and regulates the transcription of the downstream genes. CREB activity is regulation via phosphorylation by various kinases, including PKA, and Ca2+/calmodulin-dependent protein kinases. Genes known to be regulated by CREB include c-fos, BDNF, tyrosine hydroxylase, neuropeptides (such as somatostatin, enkephalin, VGF, and corticotropin-releasing hormone), as well as the circadian clock genes PER1 and PER2. CREB is critical for memory formation across a wide range of species.
~43 kDa observed.

Immunogen

Epitope: pSer133.
KLH-conjugated synthetic peptide corresponding to the amino acids including and encompassing phosphorylated Ser133 of CREB-A.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Affinity purified
Purified rabbit polyclonal antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Global Trade Item Number

SKUGTIN
06-519-AF64704055977331837

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