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Merck

07-448-S

Anti-monomethyl-Histone H3 (Lys27) Antibody, Trial Size

Upstate®, from rabbit

Synonym(s):

H3K27me1, Histone H3 (monomethyl K27), H3 histone family, member T, Histone 3, H3, Histone cluster 3, H3

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

Product Name

Anti-monomethyl-Histone H3 (Lys27) Antibody, Trial Size, Upstate®, from rabbit

biological source

rabbit

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, vertebrates

manufacturer/tradename

Upstate®

technique(s)

ChIP: suitable (ChIP-seq)
dot blot: suitable
immunocytochemistry: suitable
western blot: suitable

isotype

IgG

NCBI accession no.

NP_003484

UniProt accession no.

Q16695

shipped in

wet ice

target post-translational modification

monomethylation (Lys27)

Quality Level

100

Gene Information

human ... HIST1H3F(8968)

Physical form

Format: Purified
Protein A purified
Purified rabbit IgG in buffer containing 0.01 M phosphate buffer, pH 7.6, 0.125 M NaCl, 0.05% sodium azide and 30% glycerol.

Analysis Note

Control
HeLa acid extract
Routinely evaluated by western blot acid in extracted proteins from Hela cells.

Western Blot Analysis:
0.1-0.2 μg/mL had detected monomethylated histone H3 in acid extracted proteins from HeLa cells.

Application

Anti-monomethyl-Histone H3 (Lys27), Trial Size Antibody is a Rabbit Polyclonal Antibody for detection of Histone H3 monomethylated on lysine 27 (H3K27me1). This antibody has dot blot (DB) verified specificity and is validated in, ICC, WB, ChIP, ChIP-seq. Small pack size is ideal for screening.
Immunocytochemistry & Dot Blot:
Reported by an independent laboratory (Kohlmaier, A., 2004; Perez-Burgos, L., 2004; Peters, A. H., 2003).
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones

Biochem/physiol Actions

Broad species cross-reactivity expected, based on sequence homology.
Recognizes monomethylated histone H3 (Lys27), Mr 17 kDa.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
17 kDa

Immunogen

Epitope: Lys27
KLH-conjugated, synthetic 2X-branched peptide containing the sequence …ARmeKSA… in which meK corresponds to monomethyl-lysine at residue 27 of human histone H3.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Ashley B Reers et al.
Epigenetics & chromatin, 16(1), 25-25 (2023-06-16)
Gene expression in malaria parasites is subject to various layers of regulation, including histone post-translational modifications (PTMs). Gene regulatory mechanisms have been extensively studied during the main developmental stages of Plasmodium parasites inside erythrocytes, from the ring stage following invasion
Thea A Egelhofer et al.
Nature structural & molecular biology, 18(1), 91-93 (2010-12-07)
We have tested the specificity and utility of more than 200 antibodies raised against 57 different histone modifications in Drosophila melanogaster, Caenorhabditis elegans and human cells. Although most antibodies performed well, more than 25% failed specificity tests by dot blot

Global Trade Item Number

SKUGTIN
07-448-S04053252926273

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