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07-449-AF647

Sigma-Aldrich

Anti-trimethyl Histone H3 (Lys27), Alexa Fluor™ 647 Conjugate Antibody

0.5 mg/mL, from rabbit

Synonym(s):

Histone H3.1t, H3/t, H3t, H3/g

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

conjugate

ALEXA FLUOR™ 647

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human

species reactivity (predicted by homology)

mouse (based on 100% sequence homology)

concentration

0.5 mg/mL

technique(s)

immunocytochemistry: suitable

NCBI accession no.

NM_003493

UniProt accession no.

Q16695

shipped in

wet ice

target post-translational modification

trimethylation (Lys27)

Gene Information

human ... H3C1(8350)
mouse ... H3C1(360198)

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Histone proteins are highly post-translationally modified, and Histone H3 is the most extensively modified of the five histones. Histone H3 sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes. Di- and tri-methylation of histone H3 are gene silencing marks, while removal of these marks by the demethylase genes Jmjd1a and Jmjd2c leads to gene activation. This product is a conjugated version of Cat. No. 07-449.
~17 kDa observed (Refer to Cat. No. 07-449)

Immunogen

Linear peptide corresponding to Histone H3 trimethylated at Lys27.

Application

Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
This Anti-trimethyl Histone H3 (Lys27) antibody is validated for use in ICC for the detection of trimethyl Histone H3 (Lys27).

Physical form

Protein A purified
Purified rabbit conjugate antibody in buffer containing PBS and 15 mg/ml BSA with 0.1% Sodium Azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Immunocytochemistry Analysis: in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected trimethyl Histone H3 (Lys27) in HeLa cells.

Alexa Fluor™ is a registered trademark of Life Technologies.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Global Trade Item Number

SKUGTIN
07-449-AF64704053252971594

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