Anti-trimethyl-Histone H4 (Lys20) Antibody, Trial Size

Histones are nuclear proteins that form octameric structures which bind DNA to form units of chromatin called nucleosomes. The family of histones—H2A, H2B, H3, and H4—are key players in gene regulation. They undergo a number of post-translational modifications (PTM) in response to various stimuli, including phosphorylation on serine and threonine residues and methylation on lysine residues. PTMs produce configural changes in histone proteins that may induce nucleosome remodeling and expose or hide DNA sequences from transcriptional complexes. Histone H4 lysine 20 (H4K20) may undergo mono-, di-, or trimethylation, which is catalyzed by the methyltransferase PR-Set7 (Set8 or KMT5a). Methylated H4K20 plays a role in regulating DNA damage responses, mitosis, DNA replication, and gene expression. Trimethylation of H4K20 contributes to gene silencing, and is a mark of the repressive heterochromatin state.

~13 kDa observed. An uncharacterized band may appear at ~110 kDa in some lysates.

Epitope: Trimethylated Lys20

KLH-conjugated linear peptide corresponding to the N-terminus of human Histone H4 trimethylated at Lys20.

Anti-trimethyl-Histone H4 (Lys20) Antibody, Trial Size/ Pack is a rabbit polyclonal antibody for detection of trimethyl-Histone H4 (Lys20) also known as H4K20me3, Histone H4 (tri methyl K20) & has been validated in IP, WB, ChIP, DB.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Western Blot Analysis: 2 µg/mL from a representative lot detected trimethyl-Histone H4 (Lys20) in 10 µg of BALB3T3/A31 acid extract and RAT2 acid extract.

Dot Blot Specificity Analysis: Unmodified and various modified Histone peptides (see tables) were probed with Anti-trimethyl-Histone H4 (Lys20) (4 µg/mL).

Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated Histone H4 using chromatin from HeLa cells.

Immunoprecipitation Analysis: A representative lot immunoprecipitated trimethyl-Histone H4 (Lys20) from HeLa cell lysate.

This antibody recognizes the N-terminus of Histone H4 trimethylated at Lys20.

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
HeLa acid extract and various recombinant Histone proteins.

Evaluated by Western Blot in HeLa acid extract and various recombinant Histone proteins.

Western Blot Analysis: 2 µg/mL of this antibody detected trimethyl-Histone H4 (Lys20) in 10 µg of HeLa acid extract.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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