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14-347-M

Sigma-Aldrich

CHK2 Protein, active, 10 µg

Active, recombinant human CHK2, residues 5-543, containing N-terminal GST & C-terminal His-6 tags, for use in Kinase Assays.

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About This Item

UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.32

biological source

human

Quality Level

100

recombinant

expressed in E. coli

product line

Upstate®

specific activity

(For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.)

mol wt

Mw 89.6 kDa

purified by

(glutathione-agarose and Ni2+/NTA-agarose)

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable (kinase)

accession no.

 (FUNCTION: SwissProt: O96017 # Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on ′Ser-216′, preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at ′Thr-18′ and ′Ser-20′. )
NM_001005735.1

NCBI accession no.

NM_001005735.1
NM_007194.3
NM_145862.2

UniProt accession no.

O96017

Gene Information

human ... CHEK2(11200)

General description

CHK2
Recombinant human CHK2, residues 5-543, containing N-terminal GST and Cterminal His6 tags

Biochem/physiol Actions

Protein Target: CHK2
Target Sub-Family: CAMK

Packaging

Also available in 10 μg size and in bulk
Also available in 250 µg size --call for pricing and availability and reference catalog number 14-347M when ordering the 250 µg size.

Physical form

glutathione-agarose and Ni2+/NTA-agarose

Preparation Note

1 year at -70°C

Analysis Note

routinely evaluated by phosphorylation of the CHKtide substrate peptide

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H317

Hazard Classifications

Skin Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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S Matsuoka et al.
Science (New York, N.Y.), 282(5395), 1893-1897 (1998-12-04)
In response to DNA damage and replication blocks, cells prevent cell cycle progression through the control of critical cell cycle regulators. We identified Chk2, the mammalian homolog of the Saccharomyces cerevisiae Rad53 and Schizosaccharomyces pombe Cds1 protein kinases required for

Global Trade Item Number

SKUGTIN
14-34704053252507373

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