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14-440-M

Sigma-Aldrich

PKA Protein, Recombinant, 10 µg

Recombinant human full length PKA, catalytic subunit type alpha, untagged, for use in Kinase Assays.

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About This Item

UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.41

biological source

human

Quality Level

100

recombinant

expressed in E. coli
expressed in BL21 DE3 cells

product line

Upstate®

form

liquid

specific activity

(For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.)
8580 U/mg

shelf life

1 yr

mol wt

Mw 40.7 kDa

purified by

chromatography

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable (kinase)

solubility

water: soluble

accession no.

 (FUNCTION: SwissProt: P17612 # Phosphorylates a large number of substrates in the cytoplasm and the nucleus. )
NM_002730.3

NCBI accession no.

NM_002730.3
NM_207518.1

UniProt accession no.

P17612

storage temp.

−70°C

Gene Information

human ... PRKACA(46909581)

General description

Research area: Cell Signaling

Product Source: Expressed in E.coli BL21 (DE3) pLysS cells Recombinant human full length PKA, catalytic subunit type alpha, untagged Protein kinase A (PKA) is a serine protein kinase and heterotetrameric holoenzyme that targets serine and threonine residues.
PKA
Recombinant human full length PKA, catalytic subunit type alpha, untagged

Biochem/physiol Actions

Protein Target: PKA; Target Sub-Family: AGC. Protein kinases are vital for integrating signaling networks within eukaryotic cells. In Hedgehog-responsive cells, PKA is crucial for regulating proliferation and fate determination by influencing Hedgehog signaling transduction. PKA has numerous targets that are linked to adhesion or migration.

Packaging

Also available in 10 μg size and in bulk
Also available in 250 µg size --call for pricing and availability and reference catalog number 14-440M when ordering the 250 µg size.

Physical form

Phosphocellulose P11 chromatography

Preparation Note

1 year at -70°C

Analysis Note

routinely evaluated by phosphorylation of Kemptide substrate in vitro

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Replaces: 14-114

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protein kinase A
Morrison H
Journal of Separation Science, 173-178 (2021)
PKA: a portrait of protein kinase dynamics
Taylor SS, et al.
Biochimica et Biophysica Acta, 1697(1-2), 259-269 (2004)
Prokaryotic expression of catalytic subunit of adenosine cyclic monophosphate-dependent protein kinase.
Yonemoto, W M, et al.
Test, 200, 581-596 (1991)
Protein Kinase A Activity and Hedgehog Signaling Pathway
Kotani T
Vitamins and Hormones, 88, 273-291 (2012)
Protein Kinase A in cellular migration?Niche signaling of a ubiquitous kinase
Svec KV and Howe AK
Frontiers in molecular biosciences, 9 (2022)
View All Related Papers

Global Trade Item Number

SKUGTIN
14-44004053252588150

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