14-440-M

PKA Protein, Recombinant, 10 µg

Name: PKA Protein, Recombinant, 10 µg
Brand: MM

Recombinant human full length PKA, catalytic subunit type alpha, untagged, for use in Kinase Assays.

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About This Item

UNSPSC Code:

12352202

NACRES:

NA.41

eCl@ss:

32160405

Product Name

PKA Protein, Recombinant, 10 µg, Recombinant human full length PKA, catalytic subunit type alpha, untagged, for use in Kinase Assays.

biological source

human

recombinant

expressed in E. coli
expressed in BL21 DE3 cells

product line

Upstate^®

form

liquid

specific activity

(For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.)
8580 U/mg

shelf life

1 yr

mol wt

M_w 40.7 kDa

purified by

chromatography

species reactivity

human

manufacturer/tradename

Upstate^®

technique(s)

activity assay: suitable (kinase)

solubility

water: soluble

accession no.

(FUNCTION: SwissProt: P17612 # Phosphorylates a large number of substrates in the cytoplasm and the nucleus. )
NM_002730.3

NCBI accession no.

NM_002730.3
NM_207518.1

UniProt accession no.

P17612

storage temp.

−70°C

Quality Level

100

Gene Information

human ... PRKACA(46909581)

Analysis Note

routinely evaluated by phosphorylation of Kemptide substrate in vitro

Biochem/physiol Actions

Protein Target: PKA; Target Sub-Family: AGC. Protein kinases are vital for integrating signaling networks within eukaryotic cells. In Hedgehog-responsive cells, PKA is crucial for regulating proliferation and fate determination by influencing Hedgehog signaling transduction. PKA has numerous targets that are linked to adhesion or migration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Research area: Cell Signaling

Product Source: Expressed in E.coli BL21 (DE3) pLysS cells Recombinant human full length PKA, catalytic subunit type alpha, untagged Protein kinase A (PKA) is a serine protein kinase and heterotetrameric holoenzyme that targets serine and threonine residues.

PKA

Recombinant human full length PKA, catalytic subunit type alpha, untagged

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Replaces: 14-114

Packaging

Also available in 10 μg size and in bulk

Also available in 250 µg size --call for pricing and availability and reference catalog number 14-440M when ordering the 250 µg size.

Physical form

Phosphocellulose P11 chromatography

Preparation Note

1 year at -70°C

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protein kinase A

Morrison H

Journal of Separation Science, 173-178 (2021)

Protein Kinase A Activity and Hedgehog Signaling Pathway

Kotani T

Vitamins and Hormones, 88, 273-291 (2012)

Prokaryotic expression of catalytic subunit of adenosine cyclic monophosphate-dependent protein kinase.

Yonemoto, W M, et al.

Test, 200, 581-596 (1991)

PKA: a portrait of protein kinase dynamics

Taylor SS, et al.

Biochimica et Biophysica Acta, 1697(1-2), 259-269 (2004)

Protein Kinase A in cellular migration?Niche signaling of a ubiquitous kinase

Svec KV and Howe AK

Frontiers in molecular biosciences, 9 (2022)

View All Related Papers

Global Trade Item Number

SKU	GTIN
14-440	04053252588150

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