14-477-M

Cdk5/p35 Protein, active, 10 µg

Name: Cdk5/p35 Protein, active, 10 µg
Brand: MM

Active complex of recombinant full length human Cdk5 containing & N-terminal His6-tag & recombinant full-length human p35 containing an N-terminal GST-tag, for use in Kinase Assays.

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About This Item

UNSPSC Code:

12352202

NACRES:

NA.26

eCl@ss:

32160405

Product Name

Cdk5/p35 Protein, active, 10 µg, Active complex of recombinant full length human Cdk5 containing & N-terminal His6-tag & recombinant full-length human p35 containing an N-terminal GST-tag, for use in Kinase Assays.

biological source

human

recombinant

expressed in baculovirus infected Sf21 cells

mol wt

M_w 34 kDa (cdk5)
M_w 61 kDa (p35 with GST-tag)

manufacturer/tradename

Upstate^®

technique(s)

activity assay: suitable (kinase)

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Packaging

Also available in 250μg size as catalog # 14-477M - Call for pricing and availability.

Physical form

Ni-NTA agarose

Preparation Note

1 year at -70°C

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Analysis Note

routinely evaluated by phosphorylation of Histone H1

Biochem/physiol Actions

Protein Target: CDK5/p35

Target Sub-Family: CMGC

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Complex of recombinant fulllength human cdk5 containing and N-terminal His6-tag and recombinant full-length human p35 containing an N-terminal GST-tag

Product Source: Both are expressed by baculovirus in Sf21 insect cells

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Phosphorylation of synaptic GTPase-activating protein (synGAP) by Ca2+/calmodulin-dependent protein kinase II (CaMKII) and cyclin-dependent kinase 5 (CDK5) alters the ratio of its GAP activity toward Ras and Rap GTPases.

Ward G Walkup et al.

The Journal of biological chemistry, 290(8), 4908-4927 (2014-12-24)

synGAP is a neuron-specific Ras and Rap GTPase-activating protein (GAP) found in high concentrations in the postsynaptic density (PSD) fraction from the mammalian forebrain. We have previously shown that, in situ in the PSD fraction or in recombinant form in

A model for regulation by SynGAP-α1 of binding of synaptic proteins to PDZ-domain 'Slots' in the postsynaptic density.

Ward G Walkup et al.

eLife, 5 (2016-10-21)

SynGAP is a Ras/Rap GTPase-activating protein (GAP) that is a major constituent of postsynaptic densities (PSDs) from mammalian forebrain. Its α1 isoform binds to all three PDZ (PSD-95, Discs-large, ZO-1) domains of PSD-95, the principal PSD scaffold, and can occupy

Global Trade Item Number

SKU	GTIN
14-477M	04053252284298
14-477	04053252405983

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