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Key Documents

14-484-M

Sigma-Aldrich

PKCα Protein, active, 10 µg

Active, recombinant, full-length human PKCα, containing N-terminal His6 tag. For use in Kinase Assays.

Synonym(s):

PRKCA, Protein kinase C alpha type

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About This Item

UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.32

biological source

human

Quality Level

100

recombinant

expressed in baculovirus infected Sf21 cells

product line

Upstate®

specific activity

(For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.)

shelf life

1 yr

mol wt

Mw 78 kDa

purified by

(Ni2+/NTA-agarose)

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable (kinase)

accession no.

 (FUNCTION: SwissProt: P17252 # PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. )
NM_002737.2

NCBI accession no.

NM_002737.2

UniProt accession no.

P17252

storage temp.

−70°C

Gene Information

human ... PRKCA(5578)

General description

Research area: Apoptosis

Protein Target: PKCα

Target Sub-Family: AGC
PKCα
Recombinant, full-length human PKCα, containing N-terminal His6 tag

Application

Active, recombinant, full-length human PKCα, containing N-terminal His6 tag. For use in Kinase Assays.

Biochem/physiol Actions

Protein kinase C-α (PKCα), an isoform of protein kinase C, exhibits an anti-apoptotic and pro-proliferative function. It is commonly upregulated in tumor cells, and its depletion has been associated with apoptosis induction. The anti-apoptotic effects of PKCα are mediated through mechanisms such as phosphorylation of the anti-apoptotic protein B-cell lymphoma 2 (Bcl-2) and activation of the pro-survival protein kinase AKT. The inhibition of PKCα leads to the activation of a PKCδ-dependent apoptotic pathway in salivary epithelial cells. PKCα regulates cell cycle progression in glioma cells by increasing the expression of p21 cyclin-dependent kinase inhibitor 1 (p21 (Waf1/Cip1)), a protein involved in cell cycle regulation.

Packaging

Also available in 10 μg size and in bulk
Also available in 250 µg size --call for pricing and availability and reference catalog number 14-484M when ordering the 250 µg size.

Physical form

Ni2+/NTA-agarose

Preparation Note

1 year at -70°C

Analysis Note

routinely evaluated by phosphorylation of Histone H1.

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H317

Hazard Classifications

Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Global Trade Item Number

SKUGTIN
14-48404053252329326

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