14-734-M

FGFR1 (V561M) Protein, active, 10 µg

Name: FGFR1 (V561M) Protein, active, 10 µg
Brand: MM

Active, N-terminal GST-tagged, recombinant, human FGFR1 amino acids 456 -765 containing the V561 mutation. For use in Enzyme Assays.

Synonym(s):

Basic Fibroblast Growth Factor Receptor 1, c-fgr, CD331

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About This Item

UNSPSC Code:

12352202

NACRES:

NA.32

eCl@ss:

32160405

Product Name

FGFR1 (V561M) Protein, active, 10 µg, Active, N-terminal GST-tagged, recombinant, human FGFR1 amino acids 456 -765 containing the V561 mutation. For use in Enzyme Assays.

biological source

human

recombinant

expressed in baculovirus infected Sf21 cells

mol wt

M_w 62.3 kDa

manufacturer/tradename

Upstate^®

technique(s)

activity assay: suitable

Biochem/physiol Actions

Protein Target: FGFR1

Target Sub-Family: TK

Analysis Note

Routinely evaluated by phosphorylation of 500μM GGEEEEYFELVKKKK

Application

Research Category
Metabolism

Research Sub Category
Obesity

Metabolic Disorders

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

N-terminal GST-tagged, recombinant, human FGFR1 amino acids 456 - 765 containing the V561 mutation. The coordinates for recombinant human FGFR1 were described in Homann et al. (2001). Blencke et al. (2004) suggested mutations to a conserved threonine residue at the ATP binding site would result in inhibitor resistance. The amino acid valine 561 was mutated to a methionine in FGFR1 which corresponded to previously reported mutations found in Abl (T315) and EGFR (T766) which had been shown to confer resistance to selective inhibitors. Assay data for FGFR1 V561M had shown that this mutation had conferred resistance to PP58 (pyrido [2,3-d] pyrimidine tyrosine kinase inhibitor) compared to that of the wild type.

Product Source: Recombinant human FGFR1 expressed by baculovirus in Sf21 cells.

Other Notes

Concentration: Please see lot specific Certificarte of Analysis.

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Please see lot specific Certificarte of Analysis.

Physical form

Glutathione-agarose

Presented as a frozen solution in 50mM Tris/HCl pH 7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij 35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol.

Preparation Note

Stable for 6 months at -70°C from date of shipment. Upon first use, rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

GHS07

signalword

Warning

hcodes

H317

pcodes

P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364

Hazard Classifications

Skin Sens. 1

Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Characterization of a conserved structural determinant controlling protein kinase sensitivity to selective inhibitors.

Blencke S, Zech B, Engkvist O, Greff Z, Orfi L, Horvath Z, Keri G, Ullrich A, Daub H.

Chemistry and Biology, 5, 691-701 (2004)

Expression and purification of human recombinant GST-FGF receptor-1.

S Homann et al.

Journal of biotechnology, 86(1), 51-58 (2001-02-27)

When tumors undergo the angiogenic switch, cell growth and tissue invasion is facilitated by the formation of new capillaries from preexisting blood vessels, a process known as angiogenesis. Growth factors such as vascular endothelial growth factor/vascular permeability factor (VEGF/VPF) and

Global Trade Item Number

SKU	GTIN
14-734	04053252403682
14-734M	04053252411885

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