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Merck

14-925-M

Wee1 Protein, active, 10 µg

Active, N-terminal GST-tagged & C-terminal 6His tagged, recombinant, human Wee1, amino acids 214-end. For use in Kinase Assays.

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32
eCl@ss:
32160405

Product Name

Wee1 Protein, active, 10 µg, Active, N-terminal GST-tagged & C-terminal 6His tagged, recombinant, human Wee1, amino acids 214-end. For use in Kinase Assays.

biological source

human

recombinant

expressed in baculovirus infected Sf21 cells

mol wt

Mw 77 kDa

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable (kinase)

NCBI accession no.

NM_003390

UniProt accession no.

P30291

Quality Level

100
300

Gene Information

human ... WEE1(7465)

Analysis Note

Routinely evaluated by phosphorylation of peptide LSNLYHQGKFLQTFCGSPLYRRR.

Biochem/physiol Actions

Protein Target: WEE1

General description

Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on ′Tyr-15′. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on ′Tyr-15′ and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.
Product Source: N-terminal GST-tagged and C-terminal 6His tagged, recombinant, human Wee1 amino acids 214-end expressed by baculovirus in Sf21 insect cells.

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Packaging

Also available in 250μg size as catalog # 14-925M and in bulk as catalog # 14-925-K.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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