Anti-Citrulline (Modified) Detection Kit

Citrullination or deimination is the post-translational modification of arginine residues to citrulline residues in proteins. This process is catalyzed by enzymes called peptidylarginine deiminases (PADs).

The Anti-Citrulline (Modified) Detection Kit provides reagents for the modification and detection of citrulline-containing proteins by Western blot analysis. Modification of citrulline residues is created by a chemical reaction with 2,3-butanedione monoxime and antipyrine in a strong acid solution. Detection of the modified citrulline proteins uses a standard immunoblot protocol with an anti-Citrulline (modified) Human monoclonal antibody and a goat anti-Human IgG secondary antibody horseradish peroxidase (HRP) conjugate. This type of citrulline modification ensures the detection of citrulline residues in proteins regardless of neighboring amino acid sequences.

Kit capacity: 10 western blots

Store Anti-Modified Citrulline monoclonal antibody at 2-8°C. Store Goat Anti-Human IgG, HRP Conjugate, Reagent B, and 0.5% FeCl3 at -20°C for 4 months from date of receipt.

Anti-Modified Citrulline, clone C4 (Part number MABS487). One vial containing 100 µL of Protein A purified recombinant human monoclonal IgG1λ in PBS containing 0.05% sodium azide. Goat Anti-Human IgG, HRP Conjugate: (Part number CS216591). One vial containing 50 µL of Goat anti-Human IgG conjugated to horseradish peroxidase (HRP) in PBS, pH 7.6, with 7.5 mg/mL BSA and diluted with StabilZyme^® and glycerol to 50%. Reagent B: (Part number 20-253). One bottle containing 50 mL of 0.5% 2,3-butanedione monoxime, 0.25% antipyrine, 0.5 M acetic acid. 0.5% FeCl3: (Part number 20-255). One bottle containing 4 mL of 0.5% FeCl3. Frozen solution.

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