Key Documents

COA/COQ

AB10424

Anti-Phospho-Bad (Ser99) Antibody

Name: Anti-Phospho-Bad (Ser99) Antibody
Brand: MM

from rabbit

Synonym(s):

BCL-X/BCL-2 binding protein, BCL2-antagonist of cell death protein, BCL2-associated agonist of cell death, BCL2-binding component 6, BCL2-binding protein, Bcl-2-binding component 6, Bcl-2-like 8 protein, Bcl-XL/Bcl-2-associated death promoter

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About This Item

UNSPSC Code:

12352203

eCl@ss:

32160702

NACRES:

NA.41

biological source

rabbit

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human

technique(s)

western blot: suitable

NCBI accession no.

NP_116784

UniProt accession no.

Q92934

shipped in

wet ice

target post-translational modification

phosphorylation (pSer99)

Gene Information

human ... BAD(572)

General description

Bad is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin are found to be involved in the regulation of this protein. Bad is phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation.

18 kDa

Immunogen

This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S99 of human Bad.

Application

This Anti-Phospho-Bad (Ser99) Antibody is validated for use in WB for the detection of Phospho-Bad (Ser99).

Biochem/physiol Actions

Other species have not been tested.

The antibody reacts with the 18 kDa Phospho-Bad (Ser99) protein.

Physical form

Format: Purified

Analysis Note

Control
Western Blot : HL60 lysates.

Western Blot Analysis: 1:250 dilution of this antibody detected Phospho-Bad-S99 on HL60 lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)