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Merck

AB10520

Anti-DAP-5 Antibody

serum, from rabbit

Synonym(s):

Death-associated protein 5, aging-associated protein 1, eIF-4-gamma 2, eIF-4G 2, eIF4G 2, eukaryotic translation initiation factor 4 gamma, 2, eukaryotic translation initiation factor 4G-like 1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

conjugate

unconjugated

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, human

species reactivity (predicted by homology)

rat (based on 100% sequence homology)

technique(s)

western blot: suitable

isotype

IgG

NCBI accession no.

NP_001036024.1

UniProt accession no.

P78344

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... EIF4G2(1982)

General description

97 kDa
Death Associated Protein 5 (DAP-5) is a protein with high amino acid sequence homology to Eukaryotic Translation Initiation Factor 4G (eIF4G). Compared with eIF4G, DAP5 lacks the N-terminal region necessary for cap-dependent translation and has a unique C-terminal part functioning as a regulator for interferon-gamma induced cell death. DAP5 is cleaved at Asp790 during apoptosis . The C-terminal truncated form of DAP5 functions as a cap-independent translation initiation factor responsible for the mediation of its own translation during apoptosis.

Immunogen

Epitope: Unknown
KLH-conjugated linear peptide corresponding to human DAP-5.

Application

Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
This Anti-DAP-5 Antibody is validated for use in WB for the detection of DAP-5.

Biochem/physiol Actions

This antibody recognizes DAP-5.

Physical form

Rabbit polyclonal serum containing 0.05% sodium azide.
Unpurified

Preparation Note

Stable for 1 year at -20°C from date of receipt. Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
Jurkat cell lysate
Evaluated by Western Blot on Jurkat cell lysate.

Western Blot Analysis: 1:1,000 dilution of this antibody detected DAP-5 on 10 µg of jurkat cell lysate.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Global Trade Item Number

SKUGTIN
AB1052004053252732164

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